Human/Mouse Kir2.1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9548-100
MAB9548-SP
Detection of Human Kir2.1 by Western Blot.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Kir2.1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Kir2.1 in direct ELISAs. Detects human and mouse Kir2.1 in Immunohistochemistry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2153C
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Kir2.1
Asn352-Ile427
Accession # P63252
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
3 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
2-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Kir2.1 antibody by Western Blot. View Larger

Detection of Human Kir2.1 by Western Blot. Western blot shows lysates of Human heart ventricle tissue and human lung tissue. PVDF membrane was probed with 3 µg/mL of Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kir2.1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Kir2 by Immunohistochemistry (IHC-P).1 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

Kir2.1 in Human Heart. Kir2.1 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry Kir2 by Immunohistochemistry (IHC-Fr).1 antibody in Mouse Heart by Immunohistochemistry (IHC-Fr). View Larger

Kir2.1 in Mouse Heart. Kir2.1 was detected in perfusion fixed frozen sections of mouse heart using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intercalated discs. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry Kir2 by Immunocytochemistry (ICC).1 antibody in Human Cardiomyocytes by Immunocytochemistry (ICC). View Larger

Kir2.1 in Human Cardiomyocytes. Kir2.1 was detected in immersion fixed human cardiomyocytes using Rabbit Anti-Human/Mouse Kir2.1 Monoclonal Antibody (Catalog # MAB9548) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kir2.1

Human Inward Rectifier Potassium Channel 2 (KIR2.1), also known as KCNJ2, is a 427 amino acid (aa) integral membrane protein with 98% homology with mouse and rat KIR2.1.   This potassium channel preferentially allows potassium to flow into the cell, which is thought to generate action potential waveform and excitability in neuronal and muscle tissues. Mutations in the KIR2.1 gene are associated with Andersen syndrome, with associated paralysis, cardiac arrhythmias and dysmorphic features. KIR2.1 is widely expressed, notably in heart, placenta, lung, skeletal muscle, kidney and spleen. Expression of KIR2.1 is necessary for development of mature cardiomyocytes from mouse embryonic stem cells.

Long Name
Inward Rectifier K(+) Channel Kir2.1
Entrez Gene IDs
3759 (Human); 16518 (Mouse); 29712 (Rat)
Alternate Names
ATFB9; Cardiac inward rectifier potassium channel; HHBIRK1; HHIRK1; HIRK1; Inward rectifier K(+) channel Kir2.1; inward rectifier K+ channel KIR2.1; inward rectifier potassium channel 2; IRK1; IRK-1; IRK1LQT7; KCNJ2; Kir2.1; LQT7; Potassium channel, inwardly rectifying subfamily J member 2; potassium inwardly-rectifying channel, subfamily J, member 2; SQT3

Product Datasheets

Citation for Human/Mouse Kir2.1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cardiomyocytes Derived from Human CardiopoieticAmniotic Fluids
    Authors: A Di Baldass, MA D'Amico, P Izzicupo, G Gaggi, S Guarnieri, MA Mariggiò, I Antonucci, B Corneo, D Sirabella, L Stuppia, B Ghinassi
    Sci Rep, 2018;8(1):12028.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse Kir2.1 Antibody
By Anonymous on 12/08/2021
Application: IF Sample Tested: Cardiac ventricular tissue Species: Mouse