Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nrf2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236

Specificity

Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Nrf2 Antibody

Detection of Human Nrf2 antibody by Western Blot.

Detection of Human Nrf2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Nrf2 antibody by Western Blot.

Detection of Mouse Nrf2 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Nrf2 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Nrf2 Antibody

Application
Recommended Usage

Western Blot

0.2-2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with MG 132 (Catalog # 1748) and RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Entrez Gene IDs

4780 (Human); 18024 (Mouse); 83619 (Rat)

Gene Symbol

NFE2L2

UniProt

Q16236

Additional Nrf2 Products

Product Documents for Human/Mouse Nrf2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Nrf2 Antibody

For research use only

Citations for Human/Mouse Nrf2 Antibody

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FAQs

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail