Human/Mouse Peroxiredoxin 5 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects endogenous human and mouse Peroxiredoxin 5 in Western blots. In Western blots, this antibody shows no cross‑reactivity with recombinant human Peroxiredoxin 1, 2, 3, or 6.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Peroxiredoxin 5
    Met1-Leu214
    Accession # P30044
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse Peroxiredoxin 5 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, A431 human epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Peroxiredoxin 5 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3774) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Peroxiredoxin 5 at approximately 17 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 5

Peroxiredoxin-5 (Prx-5 or PRDX5; also AOEB166 and thioredoxin reductase) is a 22 kDa, widely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The precursor molecule is 214 amino acids (aa) in length.  It contains an N-terminal 52 aa mitochondrial targeting (signal) sequence and a 162 aa (17 kDa) mature enzyme that shows a nuclear export signal between aa 58‑67, and a peroxisome targeting motif at aa 212‑214. There are two catalytic cysteines, one at Cys100 and a second at Cys204 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H2O2 and alkyl hydroperoxides. Prx5 can undergo cleavage after Ala52, generating two splice forms. One shows a deletion of aa 59‑147, while a second shows a deletion of aa 103‑146. Mature human Prx-5 is 78% aa identical to mouse Prx-5, with most differences noted over the first 52 amino acids.

  • Entrez Gene IDs:
    25824 (Human); 54683 (Mouse); 113898 (Rat)
  • Alternate Names:
    ACR1; ACR1Peroxisomal antioxidant enzyme; Alu corepressor 1; Alu co-repressor 1; Antioxidant enzyme B166; AOEB166; AOEB166Peroxiredoxin V; AOPP; B166; EC 1.11.1.15; Liver tissue 2D-page spot 71B; MGC117264; MGC142283; MGC142285; Peroxiredoxin 5; peroxiredoxin-5, mitochondrial; PLPThioredoxin peroxidase PMP20; PMP20; PRDX5; PRDX6; PRXV; prx-V; SBBI10; Thioredoxin reductase; TPx type VI
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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