Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PHOX2B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PHOX2B
Met1-Glu94
Accession # Q99453

Specificity

Detects human PHOX2B in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse PHOX2B Antibody

PHOX2B antibody in IMR-32 Human Cell Line by Immunocytochemistry (ICC).

PHOX2B in IMR-32 Human Cell Line.

PHOX2B was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of PHOX2B by Immunohistochemistry

Detection of PHOX2B by Immunohistochemistry

GPR4 transcript and protein expression in the retrotrapezoid nucleus (RTN). A, RNAscope multiplex in situ hybridization (ISH) labeling for Gpr4 and the RTN marker Nmb at bregma level −6.48 mm. Arrowheads indicate RTN neurons that coexpress Nmb and Gpr4; arrows indicate more dorsally located neurons with high levels of Nmb that do not express Gpr4 (Shi et al., 2017). B, C, HA immunostaining in the RTN of Gpr4HA/HA (B) and wild-type Gpr4+/+ (C) mice; RTN neurons are identified by expression of PHOX2B. D, Representative maps of PHOX2B+/HA+ cells and PHOX2B-only cells through the rostrocaudal extent of the RTN (top, bregma −5.8 to −7.08), and average distribution of HA+ cells through the RTN (bottom). Data were averaged (±SEM) from four mice; scale bar, 50 µm. Image collected and cropped by CiteAb from the following open publication (https://www.eneuro.org/lookup/doi/10.1523/ENEURO.0002-24.2024), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse PHOX2B Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed IMR-32 human neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using AF4940 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PHOX2B

PHOX2B (Paired mesoderm homeobox protein 2B; also NBPhox) is a 33-35 kDa member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314 amino acids (aa) in length. It contains one DNA binding homeobox domain (aa 98-157), and two poly-Ala sequences, one between aa 159-167, and another between aa 241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence.

Long Name

Paired-like Homeobox 2b

Alternate Names

NBPhox, PMX2B

Entrez Gene IDs

8929 (Human); 18935 (Mouse); 364152 (Rat)

Gene Symbol

PHOX2B

UniProt

Q99453 (Human)

Additional PHOX2B Products

Product Documents for Human/Mouse PHOX2B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse PHOX2B Antibody

For research use only

Citations for Human/Mouse PHOX2B Antibody

Customer Reviews for Human/Mouse PHOX2B Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse PHOX2B Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse PHOX2B Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: hiPSC-derived sympathoblasts
    Species: Human
    Verified Customer | Posted 07/28/2025
    PHOX2B was detected in 4%PFA-fixed hiPSC-derived sympathoblasts using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 5 µg/mL for 1 hour at room temperature. Cells were then stained with a Cy3-conjugated donkey anti-goat IgG secondary antibody (yellow), and counterstained with DAPI (cyan). Specific staining was localized to the nuclei.
    Human/Mouse PHOX2B Antibody AF4940

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies