Human/Mouse PHOX2B Antibody

Catalog # Availability Size / Price Qty
PHOX2B in IMR-32 Human Cell Line.
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Product Details
Citations (3)
Supplemental Products

Human/Mouse PHOX2B Antibody Summary

Species Reactivity
Human, Mouse
Detects human PHOX2B in direct ELISAs.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PHOX2B
Accession # Q99453
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry PHOX2B antibody in IMR-32 Human Cell Line by Immunocytochemistry (ICC). View Larger

PHOX2B in IMR-32 Human Cell Line. PHOX2B was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human PHOX2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4940) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PHOX2B

PHOX2B (Paired mesoderm homeobox protein 2B; also NBPhox) is a 33-35 kDa member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314 amino acids (aa) in length. It contains one DNA binding homeobox domain (aa 98-157), and two poly-Ala sequences, one between aa 159-167, and another between aa 241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence.

Long Name
Paired-like Homeobox 2b
Entrez Gene IDs
8929 (Human); 18935 (Mouse); 364152 (Rat)
Alternate Names
NBPhox; NBPhoxPhox2b; Neuroblastoma Phox; paired mesoderm homeobox 2b; paired mesoderm homeobox protein 2B; paired-like homeobox 2bNBLST2; PHOX2B homeodomain protein; PHOX2B; PMX2B; PMX2Bneuroblastoma paired-type homeobox protein

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Citations for Human/Mouse PHOX2B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. BET and CDK Inhibition Reveal Differences in the Proliferation Control of Sympathetic Ganglion Neuroblasts and Adrenal Chromaffin Cells
    Authors: J Sriha, C Louis-Bren, C Pierre-Eug, S Baulande, V Raynal, A Kramdi, I Adameyko, U Ernsberger, T Deller, O Delattre, I Janoueix-L, H Rohrer
    Cancers, 2022;14(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3
    Authors: CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Cell non-autonomous requirement of p75 in the development of geniculate oral sensory neurons
    Authors: T Tang, CR Donnelly, AA Shah, RM Bradley, CM Mistretta, BA Pierchala
    Scientific Reports, 2020;10(1):22117.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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