Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 466023
View all Pygopus-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pygopus‑1
Ala2-Ala419
Accession # Q9Y3Y4

Specificity

Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Pygopus‑1 Antibody

Detection of Human and Mouse Pygopus-1 antibody by Western Blot.

Detection of Human and Mouse Pygopus‑1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Pygopus-1 Mono-clonal Antibody (Catalog # MAB3617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse Pygopus‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB3617 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pygopus-1

Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta -catenin in the nucleus during Wnt-signaling. In particular, following beta -catenin activation and translocation to the nucleus, beta -catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta -catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.

Alternate Names

Pygo1, Pygopus1

Entrez Gene IDs

26108 (Human); 72135 (Mouse)

Gene Symbol

PYGO1

UniProt

Q9Y3Y4

Additional Pygopus-1 Products

Product Documents for Human/Mouse Pygopus‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Pygopus‑1 Antibody

For research use only

Customer Reviews for Human/Mouse Pygopus‑1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse Pygopus‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse Pygopus-1 Antibody
    Name: Dong-Hwan Kim
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 07/01/2016
    Human/Mouse Pygopus‑1 Antibody MAB3617

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies