Human/Mouse Pygopus-1 Antibody

Catalog # Availability Size / Price Qty
MAB3617
MAB3617-SP
Detection of Human and Mouse Pygopus‑1 by Western Blot.
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Human/Mouse Pygopus-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Pygopus-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 466023
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Pygopus‑1
Ala2-Ala419
Accession # Q9Y3Y4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Pygopus-1 antibody by Western Blot. View Larger

Detection of Human and Mouse Pygopus‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Pygopus-1 Mono-clonal Antibody (Catalog # MAB3617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pygopus-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pygopus-1

Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45 kDa, 419 amino acid (aa) transcriptional coactivator. It contains four domains: an N‑terminal NLS (aa 35‑41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa 340‑398). It is a nuclear protein that acts in concert with BCL-9 to retain beta -catenin in the nucleus during Wnt-signaling. In particular, following beta -catenin activation and translocation to the nucleus, beta -catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, beta -catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 shares 87% aa sequence identity with mouse Pygopus-1.

Entrez Gene IDs
26108 (Human); 72135 (Mouse)
Alternate Names
DKFZp547G0910; Pygo1; pygopus 1; pygopus homolog 1 (Drosophila); pygopus homolog 1; Pygopus1; Pygopus-1; pygopus-like protein 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse Pygopus-1 Antibody
By Dong-Hwan Kim on 07/01/2016
Application: WB Sample Tested: Cell Lysates Species: Mouse