Human/Mouse/Rat Activin A beta A subunit Antibody

Catalog # Availability Size / Price Qty
MAB3381-SP
MAB3381-500
MAB3381-100
Human/Mouse/Rat Activin A  beta A subunit Antibody in Data
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Product Details
Citations (9)
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Human/Mouse/Rat Activin A beta A subunit Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects recombinant human Activin A in ELISAs. Also recognizes Activin A precursor. The amino acid sequences of mature beta A subunits from human, mouse and rat are identical. Therefore, this antibody will also detect the mature beta A subunit from mouse and rat.
Source
Monoclonal Mouse IgG1 Clone # 69403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Activin A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Human/Mouse/Rat Activin A Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Mouse/Rat Activin A beta A subunit Biotinylated Antibody (Catalog # BAM3381)

Neutralization
Measured by its ability to neutralize Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Hemoglobin Expression Induced by Activin A and Neutralization by Human/ Mouse/Rat Activin A Antibody. View Larger

Hemoglobin Expression Induced by Activin A and Neutralization by Human/ Mouse/Rat Activin A Antibody. Recombinant Human/Mouse/ Rat Activin A (Catalog # 338-AC) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin Expression elicited by Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Activin A beta A subunit Monoclonal Antibody (Catalog # MAB3381). The ND50is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin A

Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta  subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha  subunit and one of the beta  subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).

References
  1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
  2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
  3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
  4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
  6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
  7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
  8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
  9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
  10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
  11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
  12. Onichtchouk, D. et al. (1999) Nature 401:480.
  13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
  14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
  15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
  16. Schneyer, A. et al. (2003) Endocrinology 144:1671.
Entrez Gene IDs
3624 (Human); 16323 (Mouse); 29200 (Rat)
Alternate Names
Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1

Product Datasheets

Citations for Human/Mouse/Rat Activin A beta A subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Activin A programs the differentiation of human TFH cells
    Nat Immunol, 2016;17(8):976-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Adipose Stromal Cell Contact with Endothelial Cells Results in Loss of Complementary Vasculogenic Activity Mediated by Induction of Activin A.
    Authors: Merfeld-Clauss S, Lupov I, Lu H, March K, Traktuev D
    Stem Cells, 2015;33(10):3039-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  3. Low miR-143/miR-145 Cluster Levels Induce Activin A Overexpression in Oral Squamous Cell Carcinomas, Which Contributes to Poor Prognosis.
    Authors: Bufalino A, Cervigne N, de Oliveira C, Fonseca F, Rodrigues P, Macedo C, Sobral L, Miguel M, Lopes M, Paes Leme A, Lambert D, Salo T, Kowalski L, Graner E, Coletta R
    PLoS ONE, 2015;10(8):e0136599.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
    Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
    Blood, 2011;117(19):5092-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Activin A is an anticatabolic autocrine cytokine in articular cartilage whose production is controlled by fibroblast growth factor 2 and NF-kappaB.
    Authors: Alexander S, Watt F, Sawaji Y, Hermansson M, Saklatvala J
    Arthritis Rheum., 2007;56(11):3715-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases.
    Authors: Kupershmidt L, Amit T, Bar-Am O, Youdim MB, Blumenfeld Z
    J. Neurochem., 2007;103(3):962-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Fibroblast growth factor 2 modulates transforming growth factor beta signaling in mouse embryonic fibroblasts and human ESCs (hESCs) to support hESC self-renewal.
    Authors: Greber B, Lehrach H, Adjaye J
    Stem Cells, 2006;25(2):455-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Requirement of Smad3 for mast cell growth.
    Authors: Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H
    Cell. Immunol., 2006;240(1):47-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma.
    Authors: Utsunomiya T, Sonoda H, Mimori K
    Clin. Cancer Res., 2004;10(17):5702-7.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Human/Mouse/Rat Activin A beta A subunit Antibody
By Anonymous on 07/07/2020
Application: B/N Sample Tested: Serum-free Cell Culture Supernates Species: Human

We used this INHBA (100ng/mL) neutralizing antibody as part of an antibody cocktail containing several other antibodies to neutralize INHBA present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II. The effect of cytokines in the CM (+/-Neutralizing ab Cocktail) on Endothelial (HUVEC) Cell migration was measured using Incucyte Zoom Chemotaxis Assay.


Human/Mouse/Rat Activin A beta A subunit Antibody
By Anonymous on 03/28/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

I added a sulfo tag to this antibody and used it as a detection antibody when developing an immunoassay for Activin A.