Human/Mouse/Rat alpha Actinin 2 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9830-100
MAB9830-SP

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Detection of Human, Mouse, and Rat alpha Actinin 2 by Western Blot.
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Human/Mouse/Rat alpha Actinin 2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ACTN2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2285D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human ACTN2
Accession # P35609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat alpha Actinin 2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat alpha Actinin 2 by Western Blot. Western blot shows lysates of human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, and rat skeletal muslce tissue. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha Actinin 2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Alpha Actinin 2 antibody in C2C12 mouse myoblast cell line by Immunocytochemistry (ICC). View Larger

Alpha Actinin 2 in C2C12 mouse myoblast cell line. Alpha Actinin 2 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha Actinin 2

Actinins are F-actin cross-linking proteins thought to anchor actin to a variety of intracellular structures. alpha-Actinin 2 (ACTN2) is a member of the spectrin superfamily with a predicted molecular weight of approximately 104 kDa.  It is 894 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN2.  Two splice forms exist, resulting in 2 distinct isoforms. ACTN2 homodimerizes in an anti-parallel fashion, and can also form heterodimers with ACTN3.

Long Name
Actinin alpha 2
Entrez Gene IDs
88 (Human); 11472 (Mouse); 291245 (Rat)
Alternate Names
Actinin alpha 2; actinin, alpha 2; Actinin-a2; ACTN2; alpha Actinin 2; alpha-Actinin 2; Alpha-actinin skeletal muscle isoform 2; Alpha-Actinin Skeletal Muscle; Alpha-Actinin-2; CMD1AA; F-actin cross-linking protein

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Reviews for Human/Mouse/Rat alpha Actinin 2 Antibody

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Human/Mouse/Rat alpha Actinin 2 Antibody
By Anonymous on 11/23/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Mouse embryonic stem cells Species: Mouse