Human/Mouse/Rat alpha Actinin 2 Antibody Summary
Accession # P35609
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat alpha Actinin 2 by Western Blot. Western blot shows lysates of human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, and rat skeletal muslce tissue. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for alpha Actinin 2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Alpha Actinin 2 in C2C12 mouse myoblast cell line. Alpha Actinin 2 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha Actinin 2
Actinins are F-actin cross-linking proteins thought to anchor actin to a variety of intracellular structures. alpha-Actinin 2 (ACTN2) is a member of the spectrin superfamily with a predicted molecular weight of approximately 104 kDa. It is 894 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN2. Two splice forms exist, resulting in 2 distinct isoforms. ACTN2 homodimerizes in an anti-parallel fashion, and can also form heterodimers with ACTN3.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse/Rat alpha Actinin 2 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human/Mouse/Rat alpha Actinin 2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image