Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cyclophilin B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclophilin B
Asp34-Glu216
Accession # P23284

Specificity

Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots and direct ELISAs, less than 1% cross-reactivity with recombinant human Cyclophilin A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Cyclophilin B Antibody

Detection of Human/Mouse/Rat Cyclophilin B antibody by Western Blot.

Detection of Human/Mouse/Rat Cyclophilin B by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclophilin B antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Cyclophilin B in HeLa Human Cell Line.

Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cyclophilin B antibody in HeLa Human Cell Line.

Cyclophilin B in HeLa Human Cell Line.

Cyclophilin B was detected in immersion fixed wildtype (left panel) but is not detected in Cyclophilin B knockout (right panel) HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat Cyclophilin B Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Knockout Validated

Cyclophilin B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cyclophilin B knockout HeLa cell line.

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Entrez Gene IDs

5479 (Human); 19035 (Mouse); 64367 (Rat)

Gene Symbol

PPIB

UniProt

P23284

Additional Cyclophilin B Products

Product Documents for Human/Mouse/Rat Cyclophilin B Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Cyclophilin B Antibody

For research use only

Citations for Human/Mouse/Rat Cyclophilin B Antibody

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Protocols

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