Human/Mouse/Rat DEP-1/CD148 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat DEP-1/CD148 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human DEP‑1/CD148
    Arg997-Ala1337
    Accession # Q12913
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat DEP‑1/CD148 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DEP‑1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta ‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain. Over aa 997-337, human Dep-1 shares 95% aa sequence identity with mouse  and rat Dep-1.

  • References:
    1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
    2. Sorby, M. et al. (2001) Oncogene 20:5219.
    3. Jandt, E. et al. (2003) Oncogene 22:4175.
    4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
    5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
    6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
    7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
    8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
    9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
    10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
    11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
    12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
     
  • Long Name:
    Density Enhanced Receptor Protein Tyrosine Phosphatase
  • Entrez Gene IDs:
    5795 (Human); 19271 (Mouse); 29645 (Rat)
  • Alternate Names:
    CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
    J Immunol, 2014;192(2):782-91.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor.
    Authors: Spring K, Lapointe L, Caron C, Langlois S, Royal I
    Cell Signal, 2014;26(6):1283-93.
    Species: Human
    Sample Type: Recombinant Protein
    Application: IP
  3. Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
    Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
    J. Biol. Chem., 2011;286(13):10918-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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