Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all eEF-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human eEF-2
Val300-Ser500
Accession # Q6PK56

Specificity

Detects human, mouse, and rat eEF-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat eEF‑2 Antibody

Detection of Human/Mouse/Rat eEF‑2 antibody by Western Blot.

Detection of Human/Mouse/Rat eEF‑2 by Western Blot.

Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat eEF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat eEF‑2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: eEF-2

The eukaryotic translation elongation factor-2 (eEF-2) is a GTP-binding protein that is required for the translocation step from the A site to the P site of the ribosome. Phosphorylation of eEF-2 by the eEF-2 kinase causes complete inhibition of its activity.

Long Name

Eukaryotic Translation Elongation Factor 2

Alternate Names

eEF2

Entrez Gene IDs

1938 (Human); 13629 (Mouse); 29565 (Rat)

Gene Symbol

EEF2

UniProt

Q6PK56

Additional eEF-2 Products

Product Documents for Human/Mouse/Rat eEF‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat eEF‑2 Antibody

For research use only

Citations for Human/Mouse/Rat eEF‑2 Antibody

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