Detection of Human/Mouse/Rat HO‑2/HMOX2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat HO‑2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-2/HMOX2 at approximately 38 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, the constitutively expressed heme oxygenase-2 (HO-2/HMOX2) and the inducible heme oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.
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