Human/Mouse/Rat HSP27 Antibody

Catalog # Availability Size / Price Qty
AF15801
AF15801-SP
Detection of Human and Mouse HSP27 by Western Blot.
3 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat HSP27 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat HSP27.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HSP27
Thr2-Lys205
Accession # P04792
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Knockout Validated
HSP27 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inHSP27 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse HSP27 antibody by Western Blot. View Larger

Detection of Human and Mouse HSP27 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human HSP27 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human HSP27 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 33 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human HSP27 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human HSP27 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP27

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27, also known as HSPB1, is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3.

References
  1. Gusev, N.B. et al. (2002) Biochemistry (Moscow) 67:511.
  2. Garrido, C. et al. (2001) Biochem. Biophys. Res. Commun. 286:433.
  3. Garrido, C. (2002) Cell Death Diffr. 9:483.
  4. Brvey, J-M. et al. (2000) Nat. Cell Biol. 2:645.
Long Name
Heat Shock Protein 27
Entrez Gene IDs
3315 (Human); 15510 (Mouse); 24471 (Rat)
Alternate Names
28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27

Product Datasheets

Citation for Human/Mouse/Rat HSP27 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model.
    Authors: Lin YP, Hsu ME, Chiou YY
    Proteomics, 2010;10(13):2429-43.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat HSP27 Antibody

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Human/Mouse/Rat HSP27 Antibody
By Anonymous on 11/20/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat HSP27 Antibody
By Carly Garrison on 10/31/2018
Application: Microarray Sample Tested: EDTA Plasma Species: Human