Human/Mouse/Rat HSP27 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat HSP27.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HSP27
    Thr2-Lys205
    Accession # P04792
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Knockout Validated
    HSP27 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inHSP27 knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse HSP27 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with a 42 °C heat shock for 30 minutes with a 3 hour recovery. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human HSP27 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 33 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Knockout Validated
Western Blot Shows Human HSP27Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF15801) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP27

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27, also known as HSPB1, is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3.

  • References:
    1. Gusev, N.B. et al. (2002) Biochemistry (Moscow) 67:511.
    2. Garrido, C. et al. (2001) Biochem. Biophys. Res. Commun. 286:433.
    3. Garrido, C. (2002) Cell Death Diffr. 9:483.
    4. Brvey, J-M. et al. (2000) Nat. Cell Biol. 2:645.
  • Long Name:
    Heat Shock Protein 27
  • Entrez Gene IDs:
    3315 (Human); 15510 (Mouse); 24471 (Rat)
  • Alternate Names:
    28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27
  • Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model.
      Authors: Lin YP, Hsu ME, Chiou YY
      Proteomics, 2010;10(13):2429-43.
      Species: Rat
      Sample Type: Tissue Homogenates
      Application: WB
    Isotype Controls
    Description Application Cat# Citations Images  

    Normal Goat IgG Control

    Ctrl AB-108-C 191  
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    Secondary Antibodies
    Description Application Cat# Citations Images  

    Goat IgG HRP-conjugated Antibody

    WB, Simple Western HAF109 19  
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    Goat IgG HRP-conjugated Antibody

    WB HAF017 15  
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    Goat IgG (H+L) PE-conjugated Antibody

    Flow F0107 4  
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    Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

    Flow, IHC, ICC NL001 9
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    Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

    Flow, IHC, ICC NL003 5
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    Goat IgG (H+L) APC-conjugated Antibody

    Flow F0108 6  
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    Goat IgG Horseradish Peroxidase-conjugated Antibody

    WB HAF019 6  
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    Donkey Anti-Goat IgG Antibody

    WB AF109 6
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    Donkey Anti-Goat IgG Biotinylated Antibody

    WB BAF109 3
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    Goat IgG VisUCyte HRP Polymer

    IHC VC004  
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    Goat IgG (H+L) Fluorescein-conjugated Antibody

    Flow F0109 2  
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    Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

    Flow, IHC, ICC NL002
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    Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

    WB, ELISA R-401-C-ABS
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    Chicken Anti-Goat IgG Biotinylated Antibody

    WB BAF019
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    Rabbit Anti-Goat IgG Biotinylated Antibody

    WB BAF017
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    Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

    Flow F0124
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