Detection of Human/Mouse/Rat IMP2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, Caco-2 human colorectal adenocarcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat IMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5305) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for IMP2 at approximately 62 to 68 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IMP2 (IGF-II mRNA-binding protein 2; also VICKZ family member 2) is a 62 kDa member of the RRM IMP/VICKZ family of proteins. It is expressed in oocytes, spermatogonia, Leydig cells and follicular granulosa cells. IMP2 binds to AUF1, a binding protein for the AU-rich motifs of mRNA, and facilitates the degradation of cytokine and protooncogene mRNAs. Human IMP2 is 556 amino acids (aa) in length. It contains two RNA recognition regions (aa 3‑76 and 82‑157), and four KH domains (aa 193‑532) that mediate RNA binding. There are multiple splice variants. One shows an insertion of 43 aa after Asn357, a second shows a 17 aa substitution for the N-terminal 80 aa, a third shows a combination of the prior two, and a fourth shows a 15 aa substitution for aa 414‑556. Over aa 1‑220, human IMP2 is 89% aa identical to mouse IMP2.
Insulin-like Growth Factor 2 mRNA Binding Protein 2
Entrez Gene IDs:
10644 (Human); 319765 (Mouse); 303824 (Rat)
Hepatocellular carcinoma autoantigen p62; IF2B2; IGF2BP2; IGF-II mRNA-binding protein 2; IMP2; IMP-2IGF2 mRNA-binding protein 2; IMP2VICKZ family member 2; insulin-like growth factor 2 mRNA binding protein 2; insulin-like growth factor 2 mRNA-binding protein 2; p62; VICKZ2
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