Human/Mouse/Rat LHX5 Antibody Summary
Accession # Q9H2C1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat LHX5 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LHX5 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
LHX5 in Mouse Brain. LHX5 was detected in perfusion fixed frozen sections of adult mouse brain (hippocampus) using Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1.
Citation for Human/Mouse/Rat LHX5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation
Authors: S Cruz-Molin, P Respuela, C Tebartz, P Kolovos, M Nikolic, R Fueyo, WF van Ijcken, F Grosveld, P Frommolt, H Bazzi, A Rada-Igles
Cell Stem Cell, 2017;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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