Human/Mouse/Rat LHX5 Antibody

Catalog #: AF6290
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6290
AF6290-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat LHX5 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LHX5
Leu265-Trp402
Accession # Q9H2C1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat LHX5 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LHX5 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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LHX5 in Mouse Brain. LHX5 was detected in perfusion fixed frozen sections of adult mouse brain (hippocampus) using Goat Anti-Human LHX5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6290) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LHX5

LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1.

Long Name

LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5

Entrez Gene IDs

64211 (Human); 16873 (Mouse); 124451 (Rat)

Alternate Names

LHX5; LIM homeobox 5; LIM homeobox protein 5; LIM/homeobox protein Lhx5; MGC129689

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat LHX5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation
Authors: S Cruz-Molin, P Respuela, C Tebartz, P Kolovos, M Nikolic, R Fueyo, WF van Ijcken, F Grosveld, P Frommolt, H Bazzi, A Rada-Igles
Cell Stem Cell, 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot