Human/Mouse/Rat MIF Antibody
Human/Mouse/Rat MIF Antibody Summary
Pro2-Ala115
Accession # AAA36315
Complete Your Research
Complete Your Experiment
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human MIF by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of Mouse and Rat MIF by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of Human MIF by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for MIF at approximately 12 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MIF
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs non-classically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively.
- Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
- Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
- Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
- Kozak, C.A. et al. (1995) Genomics 27:405.
- Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
- Paralkar, V. and G. Wistow (1994) Genomics 19:48.
- Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
- Flieger, O. et al. (2003) FEBS Lett. 551:78.
- Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
- Sun, H-W. et al. (1996) Protein Eng. 9:631.
- Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
- Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
- Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
- Bernhagen, J. et al. (1994) Biochemistry 33:14144.
- Leng, L. et al. (2003) J. Exp. Med. 197:1467.
- Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Product Datasheets
Citations for Human/Mouse/Rat MIF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Applications: Affinity Assay -
An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection
Authors: J Lang, Y Cheng, A Rolfe, C Hammack, D Vera, K Kyle, J Wang, TB Meissner, Y Ren, C Cowan, H Tang
Stem Cell Reports, 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Targeting the MIF/CXCR7/AKT Signaling Pathway in Castration-resistant Prostate Cancer
Authors: L Jia, S Rafiei, B Gui, J Wu, XS Liu, AS Kibel
Mol. Cancer Res., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
Cytokine, 2014;69(1):47-55.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age.
Authors: de Oliveira Gomes A, de Oliveira Silva DA, Silva NM, de Freitas Barbosa B, Franco PS, Angeloni MB, Fermino ML, Roque-Barreira MC, Bechi N, Paulesu LR, Dos Santos MC, Mineo JR, Ferro EA
Am. J. Pathol., 2012;178(6):2792-801.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs.
Authors: Fonsato V, Collino F, Herrera M, Cavallari C, Deregibus M, Cisterna B, Bruno S, Romagnoli R, Salizzoni M, Tetta C, Camussi G
Stem Cells, 2012;30(9):1985-98.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
J. Immunol., 2010;185(9):5236-46.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
Cancer Lett., 2010;290(1):49-57.
Species: Human
Sample Types: Serum
Applications: Western Blot -
Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor.
Authors: Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS
Endocrinology, 2008;149(11):5747-57.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S
J. Biol. Chem., 2006;281(37):27653-61.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
Authors: Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V
Biol. Reprod., 2005;72(4):949-53.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
Authors: Chuang CC, Hung CJ, Tsai MC, Chuang YC
Crit. Care Med., 2004;32(3):734-9.
Species: Human
Sample Types: Serum
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Mouse/Rat MIF Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Human/Mouse/Rat MIF Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
This polyclonal was paired up with the monoclonal MAB289 to build a sandwich ELISA for the measurement of human MIF in serum and plasma samples.
