|Detection of Human and Mouse MKP‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MKP‑3 Monoclonal Antibody (Catalog # MAB3576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MKP‑3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
MAP Kinase Phosphatase 3 (MKP-3), also called PYST1 and Dual Specificity Phosphatase 6 (DUSP6), dephosphorylates serine, threonine, and tyrosine residues in proteins. On Western blots, MKP-3 has an apparent molecular weight of 42 kilodaltons. It has a high degree of substrate selectivity for dephosphorylating ERK kinases over stress-related kinases such as p38 and JNK. MKP-3 is ubiquitinylated prior to degradation in the proteosome. The rate of MKP-3 degradation is enhanced by ERK-mediated phosphorylation.
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