Human/Mouse/Rat Neurogranin Antibody

• Species Reactivity
  Human, Mouse, Rat
• Specificity
  Detects human, mouse, and rat Neurogranin Western blots.
• Source
  Polyclonal Sheep IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human Neurogranin
  Met1-Asp78
  Accession # Q92686
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.5 µg/mL
  See below
• Immunohistochemistry
  5-15 µg/mL
  See below

Western Blot click image to view larger

Detection of Human, Mouse, and Rat Neurogranin by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, human brain (motor cortex) tissue, and rat brain (cortex) tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogranin at approximately 14-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry click image to view larger

Neurogranin in Rat Brain. Neurogranin was detected in perfusion fixed frozen sections of rat brain (hippocampus) using Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7947) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to pyramidal neurons in the hippocampus. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reconstitution
  Sterile PBS to a final concentration of 0.2 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Entrez Gene IDs:
  4900 (Human); 64011 (Mouse); 64356 (Rat)

• Alternate Names:
  calmodulin-binding protein; hng; neurogranin (protein kinase C substrate, RC3); Neurogranin; Ng; NRGN; protein kinase C substrate; RC3; RC3ng

FAQs

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