Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 492413
View all Nrf1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Nrf1
Met1-Lys742
Accession # AAH10623

Specificity

Detects endogenous human, mouse and rat Nrf1 at 85 kDa in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Nrf1 Antibody

Detection of Human, Mouse, and Rat Nrf1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Nrf1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf1 Monoclonal Antibody (Catalog # MAB5306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Rat Nrf1 by Western Blot

Detection of Rat Nrf1 by Western Blot

Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Nrf1 by Western Blot

Detection of Rat Nrf1 by Western Blot

Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Nrf1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3‑Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nrf1

NF-E2-related factor 1 (Nrf1; also transcription factor 11 and LCR-F1) is a ubiquitously expressed transcription factor and member of the bZIP family and the CNC subfamily. Human Nrf1 is 772 amino acids (aa) in length. Like other members of the bZIP family, Nrf1 contains heptad repeats of leucine and hydrophobic residues within a putative amphipathic helical domain of 40 aa and is preceded by a 30 aa domain rich in arginine and lysine residues. A splicing variant produces two isoforms for Nrf1. Isoform 2 has a 30 aa deletion corresponding to aa 242‑271 in isoform 1. Human Nrf1 is 96% and 93% identical to bovine and mouse Nrf1, respectively.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Entrez Gene IDs

4779 (Human); 18023 (Mouse); 360610 (Rat)

Gene Symbol

NFE2L1

UniProt

AAH10623

Additional Nrf1 Products

Product Documents for Human/Mouse/Rat Nrf1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Nrf1 Antibody

For research use only

Citations for Human/Mouse/Rat Nrf1 Antibody

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