Human/Mouse/Rat Nrf1 Antibody
Human/Mouse/Rat Nrf1 Antibody Summary
Met1-Lys742
Accession # AAH10623
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Nrf1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf1 Monoclonal Antibody (Catalog # MAB5306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf1
NF-E2-related factor 1 (Nrf1; also transcription factor 11 and LCR-F1) is a ubiquitously expressed transcription factor and member of the bZIP family and the CNC subfamily. Human Nrf1 is 772 amino acids (aa) in length. Like other members of the bZIP family, Nrf1 contains heptad repeats of leucine and hydrophobic residues within a putative amphipathic helical domain of 40 aa and is preceded by a 30 aa domain rich in arginine and lysine residues. A splicing variant produces two isoforms for Nrf1. Isoform 2 has a 30 aa deletion corresponding to aa 242‑271 in isoform 1. Human Nrf1 is 96% and 93% identical to bovine and mouse Nrf1, respectively.
Product Datasheets
Citations for Human/Mouse/Rat Nrf1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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GATA3 induces mitochondrial biogenesis in primary human CD4+ T cells during DNA damage
Authors: LA Callender, J Schroth, EC Carroll, C Garrod-Ket, LEL Romano, E Hendy, A Kelly, P Lavender, AN Akbar, JP Chapple, SM Henson
Nature Communications, 2021;12(1):3379.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats.
Authors: Zou D, Liu P, Chen K, Xie Q, Liang X, Bai Q, Zhou Q, Liu K, Zhang T, Zhu J, Mi M
PLoS ONE, 2015;10(4):e0124727.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
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