Human/Mouse/Rat Orexin A/Hypocretin-1 Antibody

MAB763 11 Citations Datasheet / COA / SDS

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MAB763-SP
MAB763-500
MAB763-100

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Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Orexin A/Hypocretin-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Orexin A/Hypocretin‑1. In dot blots, no cross-reactivity with recombinant human Orexin B is observed.

Source

Monoclonal Mouse IgG₁ Clone # 97505

Purification

Protein A or G purified from ascites

Immunogen

KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of rat brain (hypothalamus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin A/Hypocretin-1

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).

References

Sakurai, T. et al. (1998) Cell 92:573.

Entrez Gene IDs

3060 (Human); 25723 (Rat)

Alternate Names

HCRT; Hcrt1; hypocretin (orexin) neuropeptide precursor; Hypocretin1; Hypocretin-1; NRCLP1; Orexin A; orexin; OXHcrt; PPORX; PPOXHypocretin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Orexin A/Hypocretin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
Authors: K Ogawa, H Suga, C Ozone, M Sakakibara, T Yamada, M Kano, K Mitsumoto, T Kasai, Y Kodani, H Nagasaki, N Yamamoto, D Hagiwara, M Goto, R Banno, Y Sugimura, H Arima
Sci Rep, 2018;8(1):3615.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
Authors: G Liguori, C Squillacio, L Assisi, A Pelagalli, A Vittoria, A Costagliol, N Mirabella
BMC Vet. Res., 2018;14(1):55.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC
Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)
Authors: G Liguori, C Squillacio, L Assisi, N Mirabella, E Langella, A Costagliol, A Vittoria
Reprod. Domest. Anim, 2017;0(0):.
Species: Alpaca
Sample Types: Whole Tissue
Applications: IHC
Orexin/hypocretin system modulates amygdala-dependent threat learning through the locus coeruleus.
Authors: Sears, Robert M, Fink, Ann E, Wigestrand, Mattis B, Farb, Claudia, de Lecea, Luis, Ledoux, Joseph E
Proc Natl Acad Sci U S A, 2013;110(50):20260-5.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Endoplasmic reticulum stress in wake-active neurons progresses with aging.
Authors: Naidoo N, Zhu J, Zhu Y, Fenik P, Lian J, Galante R, Veasey S
Aging Cell, 2011;10(4):640-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
SIRT1 regulation of wakefulness and senescence-like phenotype in wake neurons.
Authors: Panossian L, Fenik P, Zhu Y
J. Neurosci., 2011;31(11):4025-36.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Hypocretins regulate the anxiogenic-like effects of nicotine and induce reinstatement of nicotine-seeking behavior.
Authors: Plaza-Zabala A, Martin-Garcia E, de Lecea L, Maldonado R, Berrendero F
J. Neurosci., 2010;30(6):2300-10.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
An essential role for orexins in emergence from general anesthesia.
Authors: Kelz MB, Sun Y, Chen J, Cheng Meng Q, Moore JT, Veasey SC, Dixon S, Thornton M, Funato H, Yanagisawa M
Proc. Natl. Acad. Sci. U.S.A., 2008;105(4):1309-14.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Genetic mapping of Foxb1-cell lineage shows migration from caudal diencephalon to telencephalon and lateral hypothalamus.
Authors: Zhao T, Szabo N, Ma J, Luo L, Zhou X, Alvarez-Bolado G
Eur. J. Neurosci., 2008;28(10):1941-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
REM sleep changes in rats induced by siRNA-mediated orexin knockdown.
Authors: Chen L, Thakkar MM, Winston S, Bolortuya Y, Basheer R, McCarley RW
Eur. J. Neurosci., 2006;24(7):2039-48.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr

Involvement of the lateral hypothalamic peptide orexin in morphine dependence and withdrawal.
Authors: Georgescu D, Zachariou V, Barrot M, Mieda M, Willie JT, Eisch AJ, Yanagisawa M, Nestler EJ, DiLeone RJ
J. Neurosci., 2003;23(8):3106-11.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr