Detection of Human/Mouse/Rat Peroxiredoxin 6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 6
Human peroxiredoxin-6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. The molecule is 224 amino acids (aa) in length and has but one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A2 activity. Thus, Prx-6 likely plays a role in phospholipid turnover. The enzyme effect is a function of Ser32 in a GWSWG motif. Human Prx-6 is 93%, 92%, 90%, and 94% aa identical to porcine, rat, mouse, and canine Prx-6, respectively.
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