Human/Mouse/Rat Peroxiredoxin 6 Antibody

Catalog # Availability Size / Price Qty
AF3490
AF3490-SP
Human/Mouse/Rat Peroxiredoxin 6 Antibody in Data
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse/Rat Peroxiredoxin 6 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat Peroxiredoxin 6 on in Western blots. In Western blots, this antibody does not cross-react with recombinant human Peroxiredoxin 1, 2, 3, or 5.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Peroxiredoxin 6
Met1-Pro224
Accession # P30041
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat Peroxiredoxin 6 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Peroxiredoxin 6 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Peroxiredoxin 6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3490) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 6 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 6

Human peroxiredoxin-6 (Prx-6) is a 26 kDa, cytosolic antioxidant enzyme that belongs to the 1-Cys class of the THP/ahpC family of proteins. The molecule is 224 amino acids (aa) in length and has but one catalytic cysteine at Cys46. Following an attack on peroxide, Cys46 is oxidized to cysteine sulfenic acid and exists in a stable conformation. Reduced, Prx-6 is a homotetramer. When activated, it apparently forms a covalently-associated homodimer. In addition to glutathione peroxidase activity, Prx-6 is also reported to demonstrate phospholipase A2 activity. Thus, Prx-6 likely plays a role in phospholipid turnover. The enzyme effect is a function of Ser32 in a GWSWG motif. Human Prx-6 is 93%, 92%, 90%, and 94% aa identical to porcine, rat, mouse, and canine Prx-6, respectively.

Entrez Gene IDs
9588 (Human); 11758 (Mouse); 94167 (Rat)
Alternate Names
1-Cys; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; aiPLA21-Cys PRX; Antioxidant protein 2; AOP2; AOP2Non-selenium glutathione peroxidase; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.-; KIAA0106Red blood cells page spot 12; Liver 2D page spot 40; MGC46173; NSGPx; NSGPx1-Cys peroxiredoxin; p29; Peroxiredoxin 6; peroxiredoxin-6; PRDX6; PRX

Product Datasheets

Citation for Human/Mouse/Rat Peroxiredoxin 6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation.
    Authors: Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, Codazzi F, Grohovaz F, Zacchetti D
    J Neuroinflammation, 2013;10(0):130.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Peroxiredoxin 6 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
66.67%
4 Star
0%
3 Star
33.33%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat Peroxiredoxin 6 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat Peroxiredoxin 6 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Peroxiredoxin 6 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Peroxiredoxin 6 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human