Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

Catalog # Availability Size / Price Qty
AF1018
AF1018-SP
Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot.
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Citations (17)
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Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing ERK1 T202/Y204 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
5 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot. View Larger

Detection of Human Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho‑ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho‑ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Phospho-ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # AB‑105‑C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol.

Immunohistochemistry ERK1/ERK2 in Rat Brain. View Larger

ERK1/ERK2 in Rat Brain.

ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. View Larger

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

References
  1. Roskoski Jr., R. (2012) Pharmacol Res. 66:105.
Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1/ERK2

Product Datasheets

Citations for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Glycans modify mesenchymal stem cell differentiation to impact the function of resulting osteoblasts
    Authors: KM Wilson, AM Jagger, M Walker, E Seinkmane, JM Fox, R Kröger, P Genever, D Ungar
    J. Cell. Sci., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Ferric citrate and ferric EDTA but not ferrous sulfate drive amphiregulin-mediated activation of the MAP kinase ERK in gut epithelial cancer cells
    Authors: NM Scheers, DIA Pereira, N Faria, JJ Powell
    Oncotarget, 2018;9(24):17066-17077.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Characterization of TRKA signaling in acute myeloid leukemia
    Authors: SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M
    Oncotarget, 2018;9(53):30092-30105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Use of non-selective ?-blockers is associated with decreased tumor proliferative indices in early stage breast cancer
    Authors: A Montoya, CN Amaya, A Belmont, N Diab, R Trevino, G Villanueva, S Rains, LA Sanchez, N Badri, S Otoukesh, A Khammanivo, D Liss, ST Baca, RJ Aguilera, EB Dickerson, A Torabi, AK Dwivedi, A Abbas, K Chambers, BA Bryan, Z Nahleh
    Oncotarget, 2017;8(4):6446-6460.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice
    Authors: BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli
    Front Cell Neurosci, 2017;11(0):313.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  6. Sirtuin 3 (SIRT3) Regulates ?-Smooth Muscle Actin (?-SMA) Production through the Succinate Dehydrogenase-G Protein-coupled Receptor 91 (GPR91) Pathway in Hepatic Stellate Cells
    J Biol Chem, 2016;291(19):10277-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  8. Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells.
    Authors: Suenaga M, Yamamoto M, Tabata S, Itakura S, Miyata M, Hamasaki S, Furukawa T
    Anticancer Res, 2013;33(4):1547-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  9. Ability of the Met kinase inhibitor crizotinib and new generation EGFR inhibitors to overcome resistance to EGFR inhibitors.
    Authors: Nanjo, Shigeki, Yamada, Tadaaki, Nishihara, Hiroshi, Takeuchi, Shinji, Sano, Takako, Nakagawa, Takayuki, Ishikawa, Daisuke, Zhao, Lu, Ebi, Hiromich, Yasumoto, Kazuo, Matsumoto, Kunio, Yano, Seiji
    PLoS ONE, 2013;8(12):e84700.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  10. Perlecan Domain V induces VEGF secretion in brain endothelial cells through integrin alpha5beta1 and ERK-dependent signaling pathways.
    Authors: Clarke D, Al Ahmad A, Lee B, Parham C, Auckland L, Fertala A, Kahle M, Shaw C, Roberts J, Bix G
    PLoS ONE, 2012;7(9):e45257.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  11. Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer.
    Authors: Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S
    Clin. Cancer Res., 2011;17(8):2260-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  12. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011;17(11):3619-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  13. Deoxygedunin, a natural product with potent neurotrophic activity in mice.
    Authors: Jang SW, Liu X, Chan CB
    PLoS ONE, 2010;5(7):e11528.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  14. CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion.
    Authors: Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S
    J. Mol. Cell. Cardiol., 2009;46(5):612-20.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  15. Long-term high glucose concentration influences Akt, ERK1/2, and PTP1B protein expression in human aortic smooth muscle cells.
    Authors: Popov D, Nemecz M, Dumitrescu M, Georgescu A, Bohmer FD
    Biochem. Biophys. Res. Commun., 2009;388(1):51-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  16. E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
    Authors: Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
    Int. J. Cancer, 2008;123(8):1741-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
    Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
    Mol. Ther., 2007;15(8):1531-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Reviews for Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody

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Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 01/12/2018
Application: WB Sample Tested: PANC-1 human pancreatic carcinoma cell line Species: Human

Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody
By Anonymous on 01/11/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human