Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1205
MAB1205-SP
Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody in Data
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Product Details
Citations (1)
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Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1006A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10-25 µg/mL
See below
Immunocytochemistry
10-35 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. View Larger

Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-JNK (T183/Y185) in HEK293 Human Cell Line. View Larger

Phospho-JNK (T183/Y185) in HEK293 Human Cell Line. JNK phosphorylated at T183/Y185 was detected in immersion fixed HEK293 human embryonic kidney cell line untreated (lower panel) or treated with UV radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human Phospho-JNK (T183/Y185) by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-JNK (T183/Y185) by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 J/m2ultraviolet light (UV) followed by a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-JNK (T183/Y185) at approximately 46 and 56 kDa (as indicated) using 20 μg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name
C-Jun N-terminal Kinase
Alternate Names
c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

Product Datasheets

Citation for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Isolation, structural elucidation and immuno-stimulatory properties of polysaccharides from Cuminum cyminum
    Authors: M Tabarsa, S You, K Yelithao, S Palanisamy, NM Prabhu, M Nan
    Carbohydr Polym, 2020;230(0):115636.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

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Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
By Anonymous on 08/01/2017
Application: ChIP Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line Species: Cynomolgus Monkey