Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Capsaspora owczarzaki

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1006A
View all JNK Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site

Specificity

Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Detection of Human and Mouse Phospho-JNK (T183/Y185) antibody by Western Blot.

Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-JNK (T183/Y185) antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

Phospho-JNK (T183/Y185) in HEK293 Human Cell Line.

JNK phosphorylated at T183/Y185 was detected in immersion fixed HEK293 human embryonic kidney cell line untreated (lower panel) or treated with UV radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-JNK (T183/Y185) antibody by Simple WesternTM.

Detection of Human Phospho-JNK (T183/Y185) by Simple WesternTM.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 20 J/m2ultraviolet light (UV) followed by a 30 minute recovery, loaded at 0.2 mg/mL. A specific band was detected for Phospho-JNK (T183/Y185) at approximately 46 and 56 kDa (as indicated) using 20 μg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Detection of Human JNK1/2/3 by Simple Western

Detection of Human JNK1/2/3 by Simple Western

ACPA inhibits p-Akt, induces p-JNK and affects levels of specific metabolites in NSCLC lines.a Principal component analysis (PCA) score plot: Metabolomics profiling of control and ACPA-treated A549, H1299, H358, and H838 cells. b Changes in variable importance in projection (VIP) values for 19 metabolites in A549 cells. c, d, e Changes in VIP values for 20 metabolites in H1299, H358, and H838 cells. Significantly changed metabolites (*p < 0.05, indicated by arrows) were matched to apoptotic pathways. f, g, h, i Increase and decrease in several metabolites of ACPA-treated A549, H1299, H358, and H838 cells (*p < 0.05). j Simple Western showing total Akt, p-Akt (S473), total JNK46 and JNK54 and p-JNK46 and p-JNK54 (T183/Y185) in A549 cells at 24 hours after treatment with IC50 dose of ACPA. k Relative expression levels of Akt and p-Akt for control and ACPA-treated A549 cells after normalization by total vinculin protein. l Relative expression levels of JNK (46 and 54 kDa) and p-JNK for control and ACPA-treated A549 cells after normalization by total vinculin protein. *p < 0.05, Student’s t-test. All tests were done in quadruplicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33431819), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human JNK1/2/3 by Western Blot

Detection of Human JNK1/2/3 by Western Blot

Effect of LSW treatment on TNF alpha -mediated NF-kappa B and p38/JNK signaling in EA.hy926 cells. The cells were treated with LSW (50 μM) for 18 h before TNF alpha (10 ng/mL) stimulation for 15 min, followed by the detecting the protein expression of I kappa B alpha (A), p65 (B), p38 (C), and JNK (D) by Western blotting. Protein bands of I kappa B alpha were normalized to GAPDH; bands of the phosphorylated p65, p38, and JNK were normalized to their total forms. Data were normalized to the untreated group (Untr). *, p < 0.05; **, p < 0.01; ****, p < 0.0001, ns, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36359987), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Application
Recommended Usage

Immunocytochemistry

10-35 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line treated with UV radiation

Simple Western

10-25 µg/mL
Sample: HEK293T human embryonic kidney cell line treated with ultraviolet light (UV)

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line treated with ultraviolet light (UV)

Reviewed Applications

Read 3 reviews rated 4.7 using MAB1205 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JNK

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

Additional JNK Products

Product Documents for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

For research use only

Citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Customer Reviews for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
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1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa human cervical epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 11/03/2021
    Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody MAB1205
  • Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
    Name: Ryan Patel
    Application: Chromatin Immunoprecipitation
    Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line
    Species: Cynomolgus Monkey
    Verified Customer | Posted 08/01/2017
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 10/04/2015
    p-JNK expression in MDA-MB-231 in response to carboplatin treatment.
    Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody MAB1205

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Protocols

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