Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

Catalog # Availability Size / Price Qty
AF1205
AF1205-SP
Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat p46 and p54 JNK when dually phosphorylated at sites homologous to T183/Y185 of JNK1 and JNK2, and T221/Y223 of JNK3 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human, rat, and mouse JNK1 T183/Y185 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Phospho-JNK (T183/Y185) antibody by Western Blot. View Larger

Detection of Human and Mouse Phospho-JNK (T183/Y185) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m2UV-C for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Immunohistochemistry JNK antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

JNK in Human Breast Cancer Tissue. JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Immunohistochemistry JNK antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

JNK in Human Prostate. JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human prostate array using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Phospho-JNK (T183/Y185) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-JNK (T183/Y185) by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 J/m2ultraviolet light (UV) for 30 minutes, loaded at 0.2 mg/mL. Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 56 and 46 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Western Blot Detection of Mouse JNK1/2/3 by Western Blot View Larger

Detection of Mouse JNK1/2/3 by Western Blot Effect of p38MAPK (p38) inhibition on intracellular signaling following IR. Rats were pretreated with the carrier DMSO or BIRB796 (B-796) (5 mg/kg BW) for 1 hour and subjected to 1 hour of renal ischemia followed by different time points of reperfusion (15 min, 2 days, 7 days). Kidneys were harvested at given time points of reperfusion and total tissue lysates were used to determine activation pattern of MAPKs (p38MAPK, JNK, ERK) and the downstream target of p38MAPK (MK2) by phosphorylation specific antibodies. A representative immunoblot (A) and summary graphs (B, C) are shown. Results are given as mean ± SEM (n = 3). ** p < 0.01, ***p < 0.001 vs. sham-operated group; §p < 0.01, #p < 0.001 vs. IR-15 min group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24423080), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse JNK1/2/3 by Western Blot View Larger

Detection of Mouse JNK1/2/3 by Western Blot Inhibition of JNK kinases prevents p66ShcS36 phosphorylation under oxidative stress.MEFs were treated with H2O2 (a) (1 mM, 30 min) or exposed to HR (hypoxia 90 min, reoxygenation 15 min) (b). Cell lysates were probed with the antibodies indicated to assess p66Shc phosphorylation at S36. Representative Western blots (individual experiment performed under the same experimental conditions and run on the same gel) and summary graphs from at least three independent experiments are shown. Signal intensity of control samples was arbitrarily set at 1. Mitochondrial ROS production was measured in p66Shc+/+ and p66Shc−/− MEFs after prooxidant treatment as described in Material and Methods (n ≥ 4) (c) and phospho gamma H2AX was detected by immunoblotting (d, lower panel) and summary graph is shown in above panel. SP: SP600125, JNK inhibitor, and BR: BR796, p38 inhibitor were applied 1 h prior to stress application. Original blot shown in (b) have been cropped and the full length blots are shown in the Supplementary Figure S1b Statistical analysis was performed using t-test or ANOVA (*p < 0.05 **p < 0.01, ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26868434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse JNK1/2/3 by Western Blot View Larger

Detection of Mouse JNK1/2/3 by Western Blot The impact of high glucose conditions on various down-stream signaling pathways.The status of various signaling pathways in retinal AC under various glucose conditions was determined as described in Methods. Levels of active Src (A), Akt (B), ERK (C), p38 (D), JNK1 (E), Fyn (F) and p65 (G) in retinal AC under different glucose conditions were determined by Western blot analysis from equal amounts of cell lysates. The total level for each protein is shown in the lower part of each panel. The quantitative assessment of the data for each blot is shown below the blot. Data are presented as mean ± SEM. n = 3; *p<0.05 (NG vs. HG and NG vs. NG+L-Glu). Levels of active Fyn (F) in retinal AC under different glucose conditions was determined by Western blot analysis of immunoprecipitates from equal amounts of retinal AC lysates. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0103148), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK

The c-Jun N-terminal Kinases (JNKs) are part of the MAPK (mitogen-activated protein kinase) system that transmits signals from the extracellular milieu to both the cytoplasm and nucleus of the cell. Following perturbation at the cell membrane, MEKKs/MAP3Ks are initially activated, followed by their activation of MKKs/MAP2Ks, and MKKs activation of MAPKs/MAP(1)Ks. There are three classes of MAPKs: ERKs, p38 Kinases and JNKs. JNKs are 45-55 kDa protein products of three genes which, through alternative splicing, generate up to 10 possible isoforms. The phosphorylation targets for MAPKs vary, but include p53, c-MYC, ATF2 and c-Jun, the latter molecule representing the namesake for the enzyme group. The three human JNKs share approximately 80% aa sequence identity. JNKs from human, mouse and rat all contain a conserved Met-Met-Thr(183)-Pro-Tyr(185)-Val-Val motif that undergoes dual phosphorylation by MMK4 and MMK7 to activate the different JNKs. Activated by environmental stresses and inflammatory cytokines, JNKs translocate to the nucleus where they regulate the activity of several transcription factors; including the c-Jun component of AP-1 and ATF-2.

Long Name
C-Jun N-terminal Kinase
Alternate Names
c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8JNK; protein kinase JNK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

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Citations for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70
    Authors: K Suyama, D Sakai, S Hayashi, N Qu, H Terayama, D Kiyoshima, K Nagahori, M Watanabe
    Biomedicines, 2023-03-12;11(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Changes of signaling molecules in the axotomized rat facial nucleus
    Authors: T Ishijima, K Nakajima
    Journal of chemical neuroanatomy, 2022-10-29;126(0):102179.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Novel molecular mechanisms underlying the ameliorative effect of N-acetyl-L-cysteine against ?-radiation-induced premature ovarian failure in rats
    Authors: EM Mantawy, RS Said, DH Kassem, AK Abdel-Aziz, AM Badr
    Ecotoxicol. Environ. Saf., 2020-08-29;206(0):111190.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer
    Authors: JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque
    EBioMedicine, 2020-01-03;51(0):102547.
    Species: Human
    Sample Types:
    Applications: Western Blot
  6. Effects of interleukin-17A in nucleus pulposus cells and its small-molecule inhibitors for intervertebral disc disease
    Authors: K Suyama, D Sakai, N Hirayama, Y Nakamura, E Matsushita, H Terayama, N Qu, O Tanaka, K Sakabe, M Watanabe
    J. Cell. Mol. Med., 2018-09-11;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017-07-27;8(7):e2955.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Mechanistic clues to the protective effect of chrysin against doxorubicin-induced cardiomyopathy: Plausible roles of p53, MAPK and AKT pathways
    Authors: EM Mantawy, A Esmat, WM El-Bakly, RA Salah ElDi, E El-Demerda
    Sci Rep, 2017-07-06;7(1):4795.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2014-12-08;17(1):57-67.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress.
    Authors: Shin E, Huang Q, Gurel Z, Sorenson C, Sheibani N
    PLoS ONE, 2014-07-28;9(7):e103148.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury.
    Authors: Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, Koziel K, Enthammer M, Hermann M, Sickinger S, Soleiman A, Steger C, Vallant S, Sucher R, Brandacher G, Santer P, Dragun D, Troppmair J
    Cell Commun Signal, 2014-01-14;12(0):6.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Protective effect of the poly(ADP-ribose) polymerase inhibitor PJ34 on mitochondrial depolarization-mediated cell death in hepatocellular carcinoma cells involves attenuation of c-Jun N-terminal kinase-2 and protein kinase B/Akt activation.
    Mol. Cancer, 2012-05-14;11(0):34.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma.
    Authors: Bengochea A, de Souza MM, Lefrancois L, Le Roux E, Galy O, Chemin I, Kim M, Wands JR, Trepo C, Hainaut P, Scoazec JY, Vitvitski L, Merle P
    Br. J. Cancer, 2008-06-24;99(1):143-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody

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Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Phospho-JNK (T183/Y185) Antibody
By Carly Garrison on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human