Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

Catalog # Availability Size / Price Qty
AF892
AF892-SP
Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody in Data
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Product Details
Citations (4)
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Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human RSK1 S221 site
Formulation
Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry RSK1/RSK2 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

RSK1/RSK2 in Mouse Brain. RSK1/RSK2 was detected in perfusion fixed frozen sections of mouse brain (Purkinje cells in cerebellum) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human Phospho-RSK1 (S221) and RSK2 (S227) antibody by Western Blot. View Larger

Detection of Human Phospho-RSK1 (S221) and RSK2 (S227) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF892), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S221) and RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: RSK1/RSK2

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name
Ribosomal Protein S6 Kinase II
Alternate Names
RSK1/RSK2

Product Datasheets

Citations for Human/Mouse/Rat Phospho-RSK1 (S221)/RSK2 (S227) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. ERK and RSK regulate distinct steps of a cellular program that induces transition from multicellular epithelium to single cell phenotype.
    Authors: Caslavsky J, Klimova Z, Vomastek T
    Cell Signal, 2013;25(12):2743-51.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Western Blot
  2. Yersinia virulence factor YopM induces sustained RSK activation by interfering with dephosphorylation.
    Authors: Hentschke M, Berneking L, Belmar Campos C
    PLoS ONE, 2010;5(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Molecular pharmacology and antitumor activity of PHT-427, a novel Akt/phosphatidylinositide-dependent protein kinase 1 pleckstrin homology domain inhibitor.
    Authors: Meuillet EJ, Zuohe S, Lemos R, Ihle N, Kingston J, Watkins R, Moses SA, Zhang S, Du-Cuny L, Herbst R, Jacoby JJ, Zhou LL, Ahad AM, Mash EA, Kirkpatrick DL, Powis G
    Mol. Cancer Ther., 2010;9(3):706-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning.
    Authors: Sindreu CB, Scheiner ZS, Storm DR
    Neuron, 2007;53(1):79-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Secondary Antibodies

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