Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat RSK1 when phosphorylated at S380 in Western blots.  Expected to detect RSK2, RSK3, and RSK4 phosphorylated at S386, S377, and S389, respectively, based on sequence homology.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1024A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing the human RSK1 S380 site
    Accession # Q15418
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Simple Western
    1:75 dilution
    See below
  • Immunocytochemistry
    1:10 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-RSK1 (S380) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S380) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-RSK1 (S380) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, and loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK1 (S380) at approximately 90 kDa (as indicated) using a 1:75 dilution of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Immunocytochemistry
RSK1 in HeLa Human Cell Line. RSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, unstimulated (panels B and D) or stimulated with PMA (panels A and C), using Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) at 1:10 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panels; Catalog # NL004) and counterstained with DAPI (blue, lower panels). Specific staining was localized to plasma membrances and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    • 12 months from date of receipt, -20 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 °C under sterile conditions after opening.
Background: RSK1

RSK1 (ribosomal S6 kinase 1), gene name RPS6KA1 (ribosomal protein S6 kinase alpha 1), also called p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK-activated protein kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of mitogen-activated kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Autophosphorylation on S380 allows binding of PDPK1, leading to further phosphorylation and full activation of RSK1 kinase activity. Human, mouse and rat RSK1 share 98% aa sequence identity, and are identical within the region of the peptide immunogen.

  • Long Name:
    Ribosomal Protein S6 Kinase I
  • Entrez Gene IDs:
    6195 (Human); 20111 (Mouse); 81771 (Rat)
  • Alternate Names:
    90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1
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