Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1024A
View all RSK1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human RSK1 S380 site
Accession # Q15418

Specificity

Detects human, mouse, and rat RSK1 when phosphorylated at S380 in Western blots.  Expected to detect RSK2, RSK3, and RSK4 phosphorylated at S386, S377, and S389, respectively, based on sequence homology.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

Detection of Human Phospho-RSK1 (S380) antibody by Western Blot.

Detection of Human Phospho-RSK1 (S380) by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S380) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RSK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

RSK1 in HeLa Human Cell Line.

RSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, unstimulated (panels B and D) or stimulated with PMA (panels A and C), using Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panels; Catalog # NL004) and counterstained with DAPI (blue, lower panels). Specific staining was localized to plasma membrances and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-RSK1 (S380) antibody by Simple WesternTM.

Detection of Human Phospho-RSK1 (S380) by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, and loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK1 (S380) at approximately 90 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with PMA

Simple Western

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line treated with PMA or PMA and Ionomycin

Reviewed Applications

Read 1 review rated 5 using MAB79671 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RSK1

RSK1 (ribosomal S6 kinase 1), gene name RPS6KA1 (ribosomal protein S6 kinase alpha 1), also called p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK-activated protein kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of mitogen-activated kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Autophosphorylation on S380 allows binding of PDPK1, leading to further phosphorylation and full activation of RSK1 kinase activity. Human, mouse and rat RSK1 share 98% aa sequence identity, and are identical within the region of the peptide immunogen.

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Entrez Gene IDs

6195 (Human); 20111 (Mouse); 81771 (Rat)

Gene Symbol

RPS6KA1

UniProt

Q15418

Additional RSK1 Products

Product Documents for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

For research use only

Citations for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody

Customer Reviews for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody (1)

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  • Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 11/30/2022
    Mouse bone marrow stromal cells were lysed with NP-40 buffer for protein extraction. 10 ug of proteins were loaded in a 10% Acrylamide gel.
    Human/Mouse/Rat Phospho-RSK1 (S380) Antibody MAB79671

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Protocols

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