|Detection of Human/Mouse/Rat PKC gamma by Western Blot. Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5130) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
PKC gamma (protein kinase C-gamma) is a 78-80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++-activated and phospholipid-dependent neuronal enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC gamma is 697 amino acids (aa) in length. It contains two general regions: a lipid and Ca++-binding regulatory region (aa 35‑150 and 170‑260, respectively), and an ATP-binding catalytic region (aa 351‑614). PDK1-mediated phosphorylation of PKC gamma on Thr514 activates the enzyme. In addition, oxidative stress also mediates activation by inducing interchain disulfide-bond homodimerization. Over aa 621‑697, human PKC gamma is 99% and 95% aa identical to mouse and canine PKC gamma, respectively. PKC gamma is predominantly expressed in brain tissue.
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