PLC-beta 4 (phospholipase C-beta 4; also PLCretA) is a 130 kDa member of the beta -type PLC isozyme family. It is notably expressed in cerebellum and retina. PLC-beta 4 is associated with rhodopsin family GPCRs, where activation of Gq alpha subunits initiates PLC-beta 4 hydrolysis of PIP2. Human PLC-beta 4 is 1175 amino acids (aa) in length. It contains a catalytic X-box (aa 313‑463) and Y-box (aa 565‑681), followed by a calcium-binding C2 domain (aa 688‑786). There are multiple isoforms. One shows an alternate start site at Met114, a second contains a 40 aa substitution for the C-terminal 22 aa, a third shows a 14 aa substitution for the N-terminal 167 aa, and other forms demonstrate combinations of these alternative splice forms. Over aa 75‑250, human PLC-beta 4 is 97% aa identical to mouse PLC-beta 4.
Human/Mouse/Rat PLC‑ beta 4 Antibody
R&D Systems | Catalog # AF5128
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Lys75-Gln250
Accession # Q15147
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat PLC‑ beta 4 Antibody
Detection of Human/Mouse/Rat PLC-beta 4 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC-beta 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5128) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PLC-beta 4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse/Rat PLC‑ beta 4 Antibody
Western Blot
Sample: DU145 human prostate carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and Neuro-2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLC-beta 4
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PLC-beta 4 Products
Product Documents for Human/Mouse/Rat PLC‑ beta 4 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat PLC‑ beta 4 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
