Human/Mouse/Rat PP1 Catalytic Subunit Antibody

Catalog # Availability Size / Price Qty
MAB3000
MAB3000-SP

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Detection of Human/Mouse/Rat PP1ca by Western Blot.
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Product Details
Citations (2)
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Human/Mouse/Rat PP1 Catalytic Subunit Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PP1 alpha. Based on the amino acid homologies, the antibody is expected to cross‑react with PP1 beta and PP1 gamma.
Source
Monoclonal Mouse IgG2A Clone # 319319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PP1 alpha catalytic subunit
Lys41-Pro298
Accession # P62136
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PP1ca antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PP1ca by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Nb2-11 rat lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PP1 Catalytic Subunit Monoclonal Antibody (Catalog # MAB3000) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PP1 catalytic subunits at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP1

Protein Phosphatase 1, also known as PP1 and PPP1, is an enzyme that removes phosphate groups attached to serine or threonine residues in proteins. The holoenzyme is composed of two subunits, a catalytic subunit (PP1ca) that is highly conserved throughout evolution, and a wide variety of regulatory subunits that target the enzyme to specific subcellular compartments and proteins. The catalytic subunit is ubiquitously expressed in tissues and cell lines.

Long Name
Protein Phosphatase 1
Entrez Gene IDs
5499 (Human); 19045 (Mouse); 24668 (Rat)
Alternate Names
PP1; PP1A;PP-1A;PP1alpha;PPP1A;PPP1CA;Serine/threonine-protein phosphatase;Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

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Citations for Human/Mouse/Rat PP1 Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cell cycle dependent association of EBP50 with protein phosphatase 2A in endothelial cells.
    Authors: Boratko A, Gergely P, Csortos C
    PLoS ONE, 2012-04-16;7(4):e35595.
    Species: Bovine
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. TIMAP is a positive regulator of pulmonary endothelial barrier function.
    Authors: Csortos C, Czikora I, Bogatcheva NV, Adyshev DM, Poirier C, Olah G, Verin AD
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-06-27;295(3):L440-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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