Detection of Human/Mouse/Rat RACK1 by Western Blot.
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH‑1 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RACK1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RACK1 in HeLa Human Cell Line.
RACK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The receptor for activated C kinase 1 (RACK1) is highly conserved and has homology to the beta subunit of heterotrimeric G proteins. Initially isolated by its binding of active PKC isoforms, RACK1 is a scaffold protein that promotes the formation of multiprotein complexes that integrate distinct signaling pathways. RACK1 is also a 40S ribosomal subunit constituent, potentially linking these pathways directly to protein translation.
Receptor for Activated Protein Kinase C 1
Entrez Gene IDs:
10399 (Human); 14694 (Mouse); 83427 (Rat)
GNB2L1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding; RACK1; Receptor for Activated C Kinase 1; Receptor for activated C kinase
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