Detection of Human/Mouse/Rat ROCK2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, DA3 mouse myeloma cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ROCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ROCK2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92‑354), a coiled-coil region (aa 429‑1131) and a zinc-finger region (aa 1260‑1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids. Over aa 641‑846, human ROCK2 shares 94% and 96% aa identity with mouse and canine ROCK2, respectively.
Rho-associated, Coiled-coil Containing Protein Kinase 2
Entrez Gene IDs:
9475 (Human); 19878 (Mouse); 25537 (Rat)
coiled-coil-containing protein kinase 2; EC 2.7.11; EC 188.8.131.52; KIAA0619; p164 ROCK-2; Rho kinase 2; rho-associated protein kinase 2; Rho-associated, coiled-coil containing protein kinase 2; ROCK2; ROCK-II; ROKalpha
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