Human/Mouse/Rat ROCK2 Antibody

Name: Human/Mouse/Rat ROCK2 Antibody
Brand: R&D Systems
SKU: AF4790
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF4790

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ROCK2
Ile641-Ala846
Accession # O75116

Specificity

Detects human, mouse and rat ROCK2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat ROCK2 Antibody

Detection of Human/Mouse/Rat ROCK2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, DA3 mouse myeloma cell line, CH-1 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ROCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ROCK2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat ROCK2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, DA3 mouse myeloma cell line, CH‑1 mouse B cell lymphoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ROCK2

ROCK2/II (Rho-associated protein kinase 2; also Rho-kinase alpha ) is a member of the AGC serine/threonine protein kinase family. ROCK2 is a downstream target of activated RhoA. Its activation results in myosin phosphorylation with subsequent muscle contraction. Human ROCK2 is 1388 amino acids (aa) in length, and appears to be present as a dimer. It contains a protein kinase domain (aa 92‑354), a coiled-coil region (aa 429‑1131) and a zinc-finger region (aa 1260‑1315). Two isoforms show an alternate start site at M686, and a three aa substitution for the N-terminal 444 amino acids. Over aa 641‑846, human ROCK2 shares 94% and 96% aa identity with mouse and canine ROCK2, respectively.