SOD2/Mn-SOD Antibody (349810)

R&D Systems | Catalog # MAB3419

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Porcine

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 349810
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SOD2/Mn-SOD
Lys25-Lys222
Accession # P04179

Specificity

Detects human, mouse, and rat SOD2 in Western blots. In Western blots, no cross-reactivity with recombinant human SOD1 or SOD3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOD2/Mn-SOD Antibody (349810)

Detection of Human/Mouse/Rat SOD2/Mn‑SOD antibody by Western Blot.

Detection of Human/Mouse/Rat SOD2/Mn‑SOD by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD2/Mn-SOD was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
SOD2/Mn-SOD antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

SOD2/Mn-SOD in MCF‑7 Human Cell Line.

SOD2/Mn-SOD was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
SOD2/Mn-SOD antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

SOD2/Mn‑SOD in HL‑60 Human Cell Line.

SOD2/Mn-SOD was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn-SOD Monoclonal Antibody (Catalog # MAB3419) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human SOD2/Mn-SOD by Western Blot

Detection of Human SOD2/Mn-SOD by Western Blot

FAM210B is transported into and is localized in the mitochondria. (a) Schematic representation of FAM210B domains based on the primary structure of FAM210B. (b) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged mitochondria. FAM210B-GFP, GFP sequence introduced at the C terminus of FAM210B; FAM210B (MTS)-GFP, the MTS (aa 1–47) of FAM210B was added to the GFP N terminus; FAM210B ( delta MTS)-GFP, GFP was added to the C terminus of MTS (1–47)-deleted CRIF1. Scale bar, 20 mm. (c) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged endoplasmic reticulum. (d) Western blotting analysis following subcellular fractionation of GFP-tagged human FAM210B HeLa cells. (e) Western blotting analysis following subcellular fractionation of endogenous in HeLa cells. (f) The mitochondria of HeLa cells were swollen and sonicated to disrupt membranes, washed with alkali buffer (pH 11.5) to detach loosely associated proteins from membranes, and then re-isolated by ultracentrifugation. The supernatant (Supe) and membrane fractions (Pellet) were subjected to western blotting for FAM210B, TOM20, or MnSOD. (g) Mitochondria isolated from HeLa cells were subjected to proteinase K (PK) proteolysis to digest exposed proteins, and detergent (SDS) was used to disrupt both IMMs (inner membrane of mitochondria) and OMMs (outer membrane of mitochondria). The lysates were resolved and subjected to immunoblot analyses. The submitochondrial markers used are Tom20 (OMM), Cyt C (Cytochrome c, intermembrane space), NDUFS1 (IMM), and MnSOD (mitochondrial matrix) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28594398), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human SOD2/Mn-SOD by Western Blot

Detection of Human SOD2/Mn-SOD by Western Blot

FAM210B is transported into and is localized in the mitochondria. (a) Schematic representation of FAM210B domains based on the primary structure of FAM210B. (b) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged mitochondria. FAM210B-GFP, GFP sequence introduced at the C terminus of FAM210B; FAM210B (MTS)-GFP, the MTS (aa 1–47) of FAM210B was added to the GFP N terminus; FAM210B ( delta MTS)-GFP, GFP was added to the C terminus of MTS (1–47)-deleted CRIF1. Scale bar, 20 mm. (c) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged endoplasmic reticulum. (d) Western blotting analysis following subcellular fractionation of GFP-tagged human FAM210B HeLa cells. (e) Western blotting analysis following subcellular fractionation of endogenous in HeLa cells. (f) The mitochondria of HeLa cells were swollen and sonicated to disrupt membranes, washed with alkali buffer (pH 11.5) to detach loosely associated proteins from membranes, and then re-isolated by ultracentrifugation. The supernatant (Supe) and membrane fractions (Pellet) were subjected to western blotting for FAM210B, TOM20, or MnSOD. (g) Mitochondria isolated from HeLa cells were subjected to proteinase K (PK) proteolysis to digest exposed proteins, and detergent (SDS) was used to disrupt both IMMs (inner membrane of mitochondria) and OMMs (outer membrane of mitochondria). The lysates were resolved and subjected to immunoblot analyses. The submitochondrial markers used are Tom20 (OMM), Cyt C (Cytochrome c, intermembrane space), NDUFS1 (IMM), and MnSOD (mitochondrial matrix) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28594398), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for SOD2/Mn-SOD Antibody (349810)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cells and HL‑60 human acute promyelocytic leukemia cell line

Western Blot

0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB3419 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOD2/Mn-SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2), also known as Manganese (Mn) SOD, mitochondrial SOD, and IPO-B, is an intramitochondrial 22 kDa homotetramer. Each SOD2 monomer binds one Mn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD2 shares only 23% and 17% sequence identity with SOD1 and SOD3, respectively. SOD2 is, however, well conserved from species to species and shares 90% and 87% homology with mouse and rat SOD2, respectively. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation. SOD2 is required for normal biologic function of tissues by maintaining the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Entrez Gene IDs

6648 (Human); 20656 (Mouse); 24787 (Rat)

Gene Symbol

SOD2

UniProt

P04179

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody (349810)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SOD2/Mn-SOD Antibody (349810)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for SOD2/Mn-SOD Antibody (349810)

Customer Reviews for SOD2/Mn-SOD Antibody (349810) (2)

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  • Human/Mouse/Rat SOD2/Mn-SOD Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse Lung Endothelial Cells
    Species: Mouse
    Verified Customer | Posted 11/06/2021
    SOD2/Mn-SOD Antibody (349810) MAB3419
  • Human/Mouse/Rat SOD2/Mn-SOD Antibody
    Name: Joseph Moore
    Application: Western Blot
    Sample Tested: Cartilage tissue
    Species: Mouse
    Verified Customer | Posted 11/07/2019
    antibody dilution was 1:150. SOD2 clearly expressed in mouse lysates, detected with ECL kit. Western Blot Human/Mouse/Rat beta-Actin Antibody MAB8929 was used for 2ndary
    SOD2/Mn-SOD Antibody (349810) MAB3419

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Protocols

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