XPD Antibody

R&D Systems | Catalog # AF3296

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all XPD Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XPD
Met1-Gly360
Accession # P18074

Specificity

Detects human, mouse, and rat XPD in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for XPD Antibody

Detection of Human/Mouse/Rat XPD antibody by Western Blot.

Detection of Human/Mouse/Rat XPD by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for XPD Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XPD

The Xeroderma Pigmentosum D (XPD) protein is a DNA helicase that acts as a subunit of the RNA polymerase II complex during nucleotide excision repair (NER) and transcription-coupled repair (TCR). XPD is also involved in p53-mediated apoptosis. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Long Name

Xeroderma Pigmentosum D

Alternate Names

EM9, ERCC2

Entrez Gene IDs

2068 (Human); 13871 (Mouse); 308415 (Rat)

Gene Symbol

ERCC2

UniProt

P18074

Additional XPD Products

Product Documents for XPD Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for XPD Antibody

For research use only

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