Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all XPD Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human XPD
Met1-Gly360
Accession # P18074

Specificity

Detects human, mouse, and rat XPD in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat XPD Antibody

Detection of Human/Mouse/Rat XPD antibody by Western Blot.

Detection of Human/Mouse/Rat XPD by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat XPD Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XPD

The Xeroderma Pigmentosum D (XPD) protein is a DNA helicase that acts as a subunit of the RNA polymerase II complex during nucleotide excision repair (NER) and transcription-coupled repair (TCR). XPD is also involved in p53-mediated apoptosis. XP patients exhibit hypersensitivity to ultraviolet light and have a heightened incidence of skin cancer.

Long Name

Xeroderma Pigmentosum D

Alternate Names

EM9, ERCC2

Entrez Gene IDs

2068 (Human); 13871 (Mouse); 308415 (Rat)

Gene Symbol

ERCC2

UniProt

P18074

Additional XPD Products

Product Documents for Human/Mouse/Rat XPD Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat XPD Antibody

For research use only

Citations for Human/Mouse/Rat XPD Antibody

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