Human/Mouse SLAIN1 Antibody

Catalog # Availability Size / Price Qty
AF5638
AF5638-SP
Human/Mouse SLAIN1 Antibody in Data
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Human/Mouse SLAIN1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SLAIN1.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse SLAIN1
Met262-Gln483
Accession # Q68FF7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse SLAIN1 by Western Blot. View Larger

Detection of Human/Mouse SLAIN1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SLAIN1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SLAIN1 in D3 Mouse Cell Line. View Larger

SLAIN1 in D3 Mouse Cell Line. SLAIN1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAIN1

SLAIN1 (SLAIN motif-containing protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiled‑coil region (aa 21‑56), a Pro-rich segment (aa 64‑160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262‑483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.

Long Name
Slain motif-containing Protein 1
Entrez Gene IDs
122060 (Human); 105439 (Mouse)
Alternate Names
SLAIN motif family, member 1; SLAIN1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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