Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SLAIN1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse SLAIN1
Met262-Gln483
Accession # Q68FF7

Specificity

Detects human and mouse SLAIN1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse SLAIN1 Antibody

Detection of Human/Mouse SLAIN1 antibody by Western Blot.

Detection of Human/Mouse SLAIN1 by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SLAIN1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SLAIN1 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

SLAIN1 in D3 Mouse Cell Line.

SLAIN1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse SLAIN1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Western Blot

1 µg/mL
Sample: C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: SLAIN1

SLAIN1 (SLAIN motif-containing protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiled‑coil region (aa 21‑56), a Pro-rich segment (aa 64‑160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262‑483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.

Long Name

Slain motif-containing Protein 1

Alternate Names

SLAIN motif family, member 1

Entrez Gene IDs

122060 (Human); 105439 (Mouse)

Gene Symbol

SLAIN1

UniProt

Q68FF7

Additional SLAIN1 Products

Product Documents for Human/Mouse SLAIN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse SLAIN1 Antibody

For research use only

Customer Reviews for Human/Mouse SLAIN1 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse SLAIN1 Antibody and earn rewards!

Have you used Human/Mouse SLAIN1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies