SLAIN1 in D3 Mouse Cell Line.
SLAIN1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SLAIN1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human/Mouse SLAIN1 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse SLAIN1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SLAIN1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
SLAIN1 (SLAIN motif-containing protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiled‑coil region (aa 21‑56), a Pro-rich segment (aa 64‑160), and one C-terminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262‑483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.
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