Human/Mouse WASP Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse WASP in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human WASP
    Lys230-Gln310
    Accession # P42768
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse and Rat WASP by Western Blot. Western blot shows lysates of NRK rat normal kidney cell, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for WASP at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
WASP in Jurkat Human Cell Line. WASP was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human/Mouse WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3070) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASP
WASP is a 54 kDa protein belonging to the WASP/SCAR/WAVE family of proteins involved in actin polymerization. It is a highly conserved protein and is expressed selectively in hematopoietic stem cell-derived lineages. WASP contains multiple domains with unique functions. Mutations in WASP are associated with diseases of the hematopoietic/immunologic system.
  • Long Name:
    Wiskott-Aldrich Syndrome Protein
  • Entrez Gene IDs:
    7454 (Human); 22376 (Mouse)
  • Alternate Names:
    IMD2THC; Thrombocytopenia 1; WAS; WASP; WiskTHC1; wiskthrombocytopenia 1 (X-linked)
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