Human MST1/STK4 Antibody

Catalog # Availability Size / Price Qty
AF4939
AF4939-SP
Detection of Human MST1/STK4 by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (4)

Human MST1/STK4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MST1/STK4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human MST2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MST1/STK4
Lys285-Asp443
Accession # Q13043
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MST1/STK4 antibody by Western Blot. View Larger

Detection of Human MST1/STK4 by Western Blot. Western blot shows lysates of human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MST1/STK4 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry MST1/STK4 antibody in SH-SY5Y Human Cell Line by Immunocytochemistry (ICC). View Larger

MST1/STK4 in SH‑SY5Y Human Cell Line. MST1/STK4 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MST1/STK4

MST1 (mammalian sterile twenty-like 1; also Ser/Thr-protein kinase 4) is a 56 kDa class II GC (germinal center) kinase that contains an STE class catalytic domain. It is ubiquitously expressed, and found in both cytoplasm and nucleus. Human MST1 is 487 amino acids (aa) in length. It contains a Ser/Thr protein kinase domain (aa 30‑281), followed by two caspase cleavage sites (Asp326 and 349), two NLS motifs (aa 361‑370 and 441‑451) and a dimerization domain that spans the C‑terminal 57 amino acids. Upon dimerization, MST1 is activated via autophosphorylation at Thr183. Two MST1 variants exist; one shows a substitution of three Lys for the C‑terminal 85 aa, while a second shows a 27 aa substitution for the C‑terminal 55 amino acids. Over aa 285‑443, human MST1 shows 94% aa identity to mouse MST1.

Long Name
Mammalian STE20-like Protein Kinase 1/Serine/Threonine Protein Kinase 4
Entrez Gene IDs
6789 (Human); 58231 (Mouse); 311622 (Rat)
Alternate Names
AI447339;AU020804;Kas-2;KRS2;MST1;STK4;YSK3; KRS2; MST1; STK4; YSK3

Product Datasheets

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Citation for Human MST1/STK4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of microRNA signature in the progression of gestational trophoblastic disease
    Authors: JR Zhao, WW Cheng, YX Wang, M Cai, WB Wu, HJ Zhang
    Cell Death Dis, 2018-01-24;9(2):94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Reviews for Human MST1/STK4 Antibody

Average Rating: 3.8 (Based on 4 Reviews)

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Human MST1/STK4 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human MST1/STK4 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human MST1/STK4 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human MST1/STK4 Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human