Human MSX2 Antibody

Catalog # Availability Size / Price Qty
MAB7917
MAB7917-SP
MSX2 in SK‑BR‑3 Human Cell Line.
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Human MSX2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MSX2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 786607
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MSX2
Ala2-Thr132
Accession # P35548
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry MSX2 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

MSX2 in SK‑BR‑3 Human Cell Line. MSX2 was detected in immersion fixed SK-BR-3 human breast cancer cell line using Mouse Anti-Human MSX2 Monoclonal Antibody (Catalog # MAB7917) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX2

MSX2, also known as HOX-8, is an approximately 35 kDa homeobox transcription factor that is a downstream target of canonical Wnt signaling. MSX2 plays an important role in the development of mineralized tissues including bone, cartilage, and teeth. It is upregulated in atherosclerotic plaques and promotes the calcification of vascular smooth muscle cells during inflammation. MSX2 suppresses the adipocytic differentiation of multipotent mesenchymal cells. MSX2 acts in mammary gland development and the initiation of female fetal germ cell meiosis. It promotes tumorigenesis of breast and pancreatic carcinomas but inhibits the survival and invasiveness of melanoma cells. Human MSX2 contains one homeodomain (aa 143-201). Within aa 1-132, human MSX2 shares 87% aa sequence identity with mouse and rat MSX2.

Long Name
Msh Homeobox 2
Entrez Gene IDs
4488 (Human); 17702 (Mouse); 25483 (Rat)
Alternate Names
CRS2; CRS2parietal foramina 1; FPP; Homeobox protein Hox-8; homeobox protein MSX-2; Hox8; HOX8msh homeobox homolog 2 (Drosophila); msh (Drosophila) homeo box homolog 2; msh homeo box 2; msh homeobox 2; msh homeobox homolog 2; MSH; MSX2; PFM; PFM1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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