Human MSX2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human MSX2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 786607
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human MSX2
    Ala2-Thr132
    Accession # P35548
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
MSX2 in SK‑BR‑3 Human Cell Line. MSX2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Mouse Anti-Human MSX2 Monoclonal Antibody (Catalog # MAB7917) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSX2
MSX2, also known as HOX-8, is an approximately 35 kDa homeobox transcription factor that is a downstream target of canonical Wnt signaling. MSX2 plays an important role in the development of mineralized tissues including bone, cartilage, and teeth. It is upregulated in atherosclerotic plaques and promotes the calcification of vascular smooth muscle cells during inflammation. MSX2 suppresses the adipocytic differentiation of multipotent mesenchymal cells. MSX2 acts in mammary gland development and the initiation of female fetal germ cell meiosis. It promotes tumorigenesis of breast and pancreatic carcinomas but inhibits the survival and invasiveness of melanoma cells. Human MSX2 contains one homeodomain (aa 143-201). Within aa 1-132, human MSX2 shares 87% aa sequence identity with mouse and rat MSX2.
  • Long Name:
    Msh Homeobox 2
  • Entrez Gene IDs:
    4488 (Human); 17702 (Mouse); 25483 (Rat)
  • Alternate Names:
    CRS2; CRS2parietal foramina 1; FPP; Homeobox protein Hox-8; homeobox protein MSX-2; Hox8; HOX8msh homeobox homolog 2 (Drosophila); msh (Drosophila) homeo box homolog 2; msh homeo box 2; msh homeobox 2; msh homeobox homolog 2; MSH; MSX2; PFM; PFM1
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