Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Mxi1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Mxi1
Ala81-Ser228
Accession # P50539

Specificity

Detects human Mxi1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) MAD1, rhMAD3, and rhMAD4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Mxi1 Antibody

Mxi1 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

Mxi1 in LNCaP Human Cell Line.

Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Mxi1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line and U937 human histiocytic lymphoma cell line treated with PMA

Reviewed Applications

Read 1 review rated 5 using AF4185 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mxi1

Mxi-1 (Max interactor 1) is a 26 kDa member of the bHLH-leucine zipper family, Myc superfamily of transcription factors. It is a widely expressed transcriptional repressor that heterodimerizes with Max and blocks Max-Myc gene activation. Human Mxi-1 is 228 amino acids (aa) in length. It contains a DNA binding motif
(aa 69‑80) and a bHLH domain (aa 81‑120). Multiple splice variants of Mxi-1 appear to exist. They may incorporate an alternate start site at Met37, a 92 aa substitution for the first 26 N-terminal amino acids, or a deletion of the C-terminal 59 or 74 amino acids. Over aa 81‑228, human Mxi-1 is 95% aa identical to mouse Mxi-1.

Long Name

MAX Interacting Protein 1

Alternate Names

MAD2, MXD2, MXI, MXI-WR

Entrez Gene IDs

4601 (Human); 17859 (Mouse); 25701 (Rat)

Gene Symbol

MXI1

UniProt

P50539

Additional Mxi1 Products

Product Documents for Human Mxi1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mxi1 Antibody

For research use only

Citations for Human Mxi1 Antibody

Customer Reviews for Human Mxi1 Antibody (1)

5 out of 5
1 Customer Rating
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  • Human Mxi1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: SK-Mel-28 human malignant melanoma cell line
    Species: Human
    Verified Customer | Posted 07/26/2021
    Human Mxi1 Antibody AF4185

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