Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2021A
View all MYH7 Primary Antibodies »

Product Specifications

Immunogen

Human MYH7 synthetic peptide
Accession # P12883

Specificity

Detects human MYH7 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human MYH7 Antibody

Detection of MYH7 antibody by Western Blot.

Detection of MYH7 by Western Blot.

Western blot shows lysates of human heart (ventricle) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MYH7 antibody in Human Cardiomyocytes by Immunocytochemistry (ICC).

MYH7 in Human Cardiomyocytes.

MYH7 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human MYH7 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes

Western Blot

0.5 µg/mL
Sample:   Human heart (ventricle) tissue

Reviewed Applications

Read 1 review rated 4 using MAB90961 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYH7

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta. Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Long Name

Myosin Heavy Chain 7, Cardiac Muscle, Beta

Alternate Names

CMD1S, CMH1, MPD1, MYHCB, Myosin-7, SPMD, SPMM

Entrez Gene IDs

4625 (Human); 140781 (Mouse); 29557 (Rat)

Gene Symbol

MYH7

UniProt

P12883

Additional MYH7 Products

Product Documents for Human MYH7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MYH7 Antibody

Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Related Research Areas

Cardiac Stem Cell Markers

Citations for Human MYH7 Antibody

Customer Reviews for Human MYH7 Antibody (1)

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  • Human MYH7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human iPSC-derived cardiomyocytes
    Species: Human
    Verified Customer | Posted 03/05/2019
    the antibody was used for immunofluorescence staining on day 15 human iPSC-derived cardiomyocytes (the day of initiation of differentiation from iPSCs is counted as day 0).
    Human MYH7 Antibody MAB90961

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Protocols

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