Human MYH7 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB90961-100
MAB90961-SP
Human MYH7 Antibody in Data
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human MYH7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MYH7 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2021A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human MYH7 synthetic peptide
Accession # P12883
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of MYH7 antibody by Western Blot. View Larger

Detection of MYH7 by Western Blot. Western blot shows lysates of human heart (ventricle) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry MYH7 antibody in Human Cardiomyocytes by Immunocytochemistry (ICC). View Larger

MYH7 in Human Cardiomyocytes. MYH7 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYH7

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta. Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Long Name
Myosin Heavy Chain 7, Cardiac Muscle, Beta
Entrez Gene IDs
4625 (Human); 140781 (Mouse); 29557 (Rat)
Alternate Names
cardiac muscle beta isoform; CMD1S; CMD1SMGC138376; CMH1; DKFZp451F047; MGC138378; MPD1; MYH7; MYHCB; MyHC-beta; myhc-slow; myopathy, distal 1; myosin heavy chain (AA 1-96); myosin, heavy chain 7, cardiac muscle, beta; myosin, heavy polypeptide 7, cardiac muscle, beta; Myosin-7; rhabdomyosarcoma antigen MU-RMS-40.7A; SPMD; SPMM

Product Datasheets

Citation for Human MYH7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease
    Authors: Y Zhao, C Wang, C Wang, X Hong, J Miao, Y Liao, L Zhou, Y Liu
    Sci Rep, 2018;8(1):8996.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MYH7 Antibody

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Human MYH7 Antibody
By Anonymous on 03/05/2019
Application: ICC/IF Sample Tested: human iPSC-derived cardiomyocytes Species: Human

the antibody was used for immunofluorescence staining on day 15 human iPSC-derived cardiomyocytes (the day of initiation of differentiation from iPSCs is counted as day 0).