Human N-Terminal Pro-BNP Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB36042-100
MAB36042-SP
Human Pro-BNP ELISA Standard Curve.
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Human N-Terminal Pro-BNP Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pro-BNP but does not detect mature human BNP peptide in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2258B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Pro-BNP
His27-His134
Accession # P16860
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9538).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human NT Pro-BNP DuoSet ELISA Kit (Catalog # DY3604-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Pro-BNP ELISA Standard Curve. Recombinant human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9538) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human N-terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNP

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa signal peptide and a 108 aa Pro-BNP (aa 27-134). Proteolytic digestion of Pro-BNP results in formation of 76 aa amino-terminal NT-proBNP (aa 27-102) and biologically active 32 aa BNP hormone molecule (aa 103-134). Both proBNP and NTpro-BNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Long Name
Brain Natriuretic Peptide
Entrez Gene IDs
4879 (Human); 18158 (Mouse); 25105 (Rat)
Alternate Names
BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB

Product Datasheets

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