Human N-Terminal Pro-BNP Antibody

MAB9538 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9538-100
MAB9538-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human N-Terminal Pro-BNP Antibody Summary

Species Reactivity

Human

Specificity

Detects human N-Terminal Pro-BNP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 370129

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human N-Terminal Pro-BNP
His27-His134
Accession # P16860

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA captureantibody when paired with Rabbit Anti-Human N-terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042).

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769).

This product is intended for assay development onvarious assay platforms requiring antibody pairs. We recommend the Human NT Pro-BNP DuoSet ELISA Kit (Catalog # DY3604-05)for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

ELISA

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Human NT Pro-BNP ELISA Standard Curve. Recombinant human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9538) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB36042) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA

View Larger

Human NT Pro-BNP ELISA Standard Curve. Recombinant Human Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro- BNP Monoclonal Antibody (Catalog # MAB9769) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNP

Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro‑BNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa 103‑134) promotes vasodilation and fluid and sodium excretion. Human BNP precursor shares 29% and 51% aa sequence identity with mouse and porcine BNP precursor, respectively.

Long Name

Brain Natriuretic Peptide

Entrez Gene IDs

4879 (Human); 18158 (Mouse); 25105 (Rat)

Alternate Names

BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB