Human N-Terminal Pro-BNP Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Human N-Terminal Pro-BNP Antibody in ELISA
1 Image
Product Details
Supplemental Products

Human N-Terminal Pro-BNP Antibody Summary

Species Reactivity
Detects human Pro-BNP but does not detect mature human BNP peptide in direct ELISAs.
Recombinant Monoclonal Mouse IgG Clone # 817130R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human Pro-BNP
Accession # P16860
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human NT Pro-BNP DuoSet ELISA Kit (Catalog # DY3604-05) for convenient development of a sandwich ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human N-Terminal Pro-BNP ELISA Standard Curve. Recombinant Human N-Terminal Pro-BNP protein was serially diluted 2-fold and captured by Mouse Anti-Human N-Terminal Pro-BNP Monoclonal Antibody (Catalog # MAB9769) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human N-Terminal Pro BNP Monoclonal Antibody (Catalog # MAB9538) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BNP

Human BNP (brain natriuretic peptide) is a 3.5 kDa, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12 kDa, 108 amino acid (aa) prohormone with a 76 aa prosegment and a 32 aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27 kDa glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP, respectively.

Long Name
Brain Natriuretic Peptide
Entrez Gene IDs
4879 (Human); 18158 (Mouse); 25105 (Rat)
Alternate Names
BNF; BNP; brain type natriuretic peptide; Gamma-brain natriuretic peptide; natriuretic peptide B; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; NPPB

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reconstitution Buffers

Secondary Antibodies

Reviews for Human N-Terminal Pro-BNP Antibody

There are currently no reviews for this product. Be the first to review Human N-Terminal Pro-BNP Antibody and earn rewards!

Have you used Human N-Terminal Pro-BNP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review