Detection of Human NEDD9/CASL by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human NEDD9/CASL Monoclonal Antibody (Catalog # MAB5459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for NEDD9/CASL at approximately 110 kDa & 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NEDD9 (also HEF1 and CASL) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Tyr-rich SH2-binding region (aa 90-350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710-760). NEDD9 usually runs at 105 or 115 kDa in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155-188, while two others show 20 or 7 aa substitutions for aa 155-834 or 1-153, respectively. Over aa 153-366, human and mouse NEDD9 share 85% aa identity.
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9
Entrez Gene IDs:
4739 (Human); 18003 (Mouse); 291044 (Rat)
Cas scaffolding protein family member 2; CAS2; CASL; CAS-LCASS2cas-like docking; CASLdJ49G10.2; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; enhancer of filamentation 1; HEF1; HEF1Crk-associated substrate related; NEDD9; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; p105; Renal carcinoma antigen NY-REN-12
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