Human NF-H Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of NF‑H in Human Brain Cerebellum. NF‑H was detected in immersion fixed paraffin-embedded sections of human brain cerebellum using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinjie neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
View Larger
Detection of NF‑H in HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells. NF‑H was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line (positive) and Raji human Burkitt's lymphoma cell line (negative) cells using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
Detection of NF‑H in K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells. NF‑H was detected in immersion fixed K562 Human Chronic Myelogenous Leukemia Cell Line (Positive) and Raji Human Burkitt's Lymphoma Cell Line (Negative) Cells using Mouse Anti-Human NF‑H Monoclonal Antibody (Catalog # MAB31083) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-H
The human Neurofilament Heavy chain, also known as NF-H and NEFH, is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. In the region used for immunization, mouse and rat NF-H are each 93% identical to human NF-H.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human NF-H Antibody
There are currently no reviews for this product. Be the first to review Human NF-H Antibody and earn rewards!
Have you used Human NF-H Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
