Human NF-H Antibody

Catalog # Availability Size / Price Qty
AF3108
AF3108-SP

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NF-H in Human Brain.
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Product Details
Citations (1)
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Human NF-H Antibody Summary

Species Reactivity
Human
Specificity
Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) NF-M and rhNF-L is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NF-H
Met1-Ala380
Accession # P12036
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Human NF-H
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry NF-H antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

NF-H in Human Brain. NF-H was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-H

Neurofilament (NF)-H is a 200 kDa neurofilament protein and is one of three NF subunits. In the protein region used for immunization, human NF-H shares approximately 92% sequence identity with the mouse or rat protein.

Long Name
Neurofilament Protein, Heavy Chain
Entrez Gene IDs
4744 (Human)
Alternate Names
200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H

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Citation for Human NF-H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications
    Authors: GD Vatine, R Barrile, MJ Workman, S Sances, BK Barriga, M Rahnama, S Barthakur, M Kasendra, C Lucchesi, J Kerns, N Wen, WR Spivia, Z Chen, J Van Eyk, CN Svendsen
    Cell Stem Cell, 2019;24(6):995-1005.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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