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Human NFIL3/E4BP4 Antibody

Catalog #: MAB11657 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB11657-100
MAB11657-SP

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Detection of Human NFIL3/E4BP4 by Western Blot.
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Human NFIL3/E4BP4 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human NFIL3 in Direct ELISA.
Source
Monoclonal Mouse IgG2A Clone # 1095812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NFIL-3
Lys140-Gly462
Accession # Q16649
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
HepG2 human hepatocellular carcinoma cell line
Simple Western
20 µg/mL
HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human NFIL3/E4BP4 by Western Blot. Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NFIL3/E4BP4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western View Larger

Detection of Human NFIL3/E4BP4 by Simple WesternTM. Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for NFIL3/E4BP4 at approximately 63 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human NFIL3/E4BP4 Monoclonal Antibody (Catalog # MAB11657). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFIL3/E4BP4

NFIL3 (Nuclear Factor, Interleukin 3 Regulated), also called E4BP4 (adenovirus E4 promoter binding protein 4), is an approximately 58 kDa transcription factor of the bZIP (basic leucine zipper) family. It is a transcriptional regulator expressed in T and pro-B lymphocytes and cardiomyocytes, binds IL-3 and promoters as a homodimer, and enhances cell survival. NFIL3 has also been shown to be involved in the development of Innate Lymphoid Cells (ILCs; 1,2). The 462 amino acid (aa) human NFIL3 contains a basic DNA binding domain (aa 73-95), a leucine zipper (aa 96-124), and a transcriptional repression domain (aa 299-363) that is potentially regulated by phosphorylation at Ser301 and Ser353. Within the region used as an immunogen, human NFIL3 shares 83% aa sequence identity with mouse and rat NFIL3.

References
  1. Seillet, C. et al. (2014) J Exp Med. 211:1733.
  2. Geiger, T.L. et al. (2014) J Exp Med. 211:1723.
Long Name
Nuclear Factor, Interleukin 3 Regulated
Entrez Gene IDs
4783 (Human); 18030 (Mouse); 114519 (Rat)
Alternate Names
E4BP4; E4BP4IL3BP1; IL3BP1; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL3; NF-IL3A; NFIL3AE4 promoter-binding protein 4; nuclear factor interleukin 3 regulated protein; nuclear factor interleukin-3-regulated protein; nuclear factor, interleukin 3 regulated; Transcriptional activator NF-IL3A

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