Human NHA2/SLC9B2 Antibody

Catalog # Availability Size / Price Qty
MAB8404
Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry.
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Human NHA2/SLC9B2 Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG2B Clone # 896151
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
Met1-Val537
Accession # NP_849155
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NHA2/SLC9B2 antibody in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP antibody by Flow Cytometry. View Larger

Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with and either (A) Mouse Anti-Human NHA2/SLC9B2 Monoclonal Antibody (Catalog # MAB8404) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NHA2/SLC9B2

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

Long Name
Sodium/Hydrogen Cation Proton Antiporter 2
Entrez Gene IDs
133308 (Human); 7086 (Mouse); 679958 (Rat)
Alternate Names
FLJ23984; Mitochondrial Na(+)/H(+) exchanger NHA2; mitochondrial sodium/hydrogen exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; Na+/H+ exchanger domain containing 2; NHA2; NHA2NHE10; NHE domain-containing protein 2; NHE10; NHEDC2; SLC9B2; Sodium/hydrogen exchanger-like domain-containing protein 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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