Human NHA2/SLC9B2 Antibody (896151)

R&D Systems | Catalog # MAB8404

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 896151
View all NHA2/SLC9B2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human NHA2/SLC9B2
Met1-Val537
Accession # NP_849155

Specificity

Detects HEK293 cells transfected with human NHA2/SLC9B2 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NHA2/SLC9B2 Antibody (896151)

Detection of NHA2/SLC9B2 antibody in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP antibody by Flow Cytometry.

Detection of NHA2/SLC9B2 in HEK293 Human Cell Line Transfected with Human NHA2/SLC9B2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP was stained with and either (A) Mouse Anti-Human NHA2/SLC9B2 Monoclonal Antibody (Catalog # MAB8404) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human NHA2/SLC9B2 Antibody (896151)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human NHA2/SLC9B2 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NHA2/SLC9B2

NHA2/SLC9B2 (Solute Carrier family 9, subfamily B, member 2) is a member of the solute carrier family of multi-pass membrane proteins. Sodium hydrogen antiporters, such as SLC9B2 (NHA2), convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. SLC9B2 contributes to organellar volume homeostasis and is required for osteoclast differentiation and bone resorption activity.

Long Name

Sodium/Hydrogen Cation Proton Antiporter 2

Alternate Names

NHE10, NHEDC2, SLC9B2

Entrez Gene IDs

133308 (Human); 7086 (Mouse); 679958 (Rat)

Gene Symbol

SLC9B2

UniProt

NP_849155

Additional NHA2/SLC9B2 Products

Product Documents for Human NHA2/SLC9B2 Antibody (896151)

Certificate of Analysis

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Product Specific Notices for Human NHA2/SLC9B2 Antibody (896151)

For research use only

Related Research Areas

Ion Channels and Regulators

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