Human Nidogen-2 Antibody

Catalog # Availability Size / Price Qty
AF3385
AF3385-SP
Nidogen‑2 in Human Kidney.
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Product Details
Citations (5)
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Human Nidogen-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nidogen-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Nidogen-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Nidogen‑2
Leu31-Lys1375 (Gly832Ala)
Accession # Q14112
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Nidogen‑2 (Catalog # 3385-ND)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Nidogen-2 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

Nidogen‑2 in Human Kidney. Nidogen-2 was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human Nidogen-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3385) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nidogen-2

Nidogen-2 (also named entactin-2) is a 200 kDa, secreted, monomeric basement membrane glycoprotein (1). Nidogens 1 and 2 are expressed in nearly all basement membranes (1‑3) where they interact with laminins, collagen type IV and proteoglycan family members to form structural scaffolds (4, 5). In mouse, Nidogens 1 and 2 appear to substitute for each other. Deletion of one nidogen gives a mild phenotype, but deletion of both nidogens is lethal (6, 7). Affinity of laminin binding is much lower for human Nidogen-2 than that of mouse Nidogen-2, indicating that human Nidogen-2 may not be a strict substitute for Nidogen-1 (1). Both nidogens bind perlecan and collagens I and IV, but only Nidogen-1 binds fibulins (1, 3). The two nidogens show approximately 50% amino acid (aa) identity in human and are structurally similar (1, 4, 6). Cleavage of a 28 aa signal sequence from human Nidogen-2 produces a 1219 aa mature protein containing three globular domains (G1‑3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 Nidogen ( beta -barrel) domain interacts with perlecan, and the C-terminal G3 beta -propeller structure is associated with laminin binding. The mucin-like link region is longer in Nidogen-2 than nidogen-1, and contains both N- and O-glycosylation (2, 8). There is one EGF‑like motif and a short peptide that ligates alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF‑like motifs, two of which bind calcium, and two thyroglobulin type 1 domains that serve as a binding site for alpha v beta 3 integrins. Mature human Nidogen-2 is 80% aa identical to both mouse and rat Nidogen-2, and 73% aa identical to both canine and bovine Nidogen-2.

References
  1. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Salmivirta, K. et al. (2002) Exp. Cell Res. 279:188.
  4. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
  5. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  6. Schymeinsky, J. et al. (2002) Mol. Cell. Biol. 22:6820.
  7. Bader, B.L. et al. (2005) Mol. Cell. Biol. 25:6846.
Entrez Gene IDs
22795 (Human); 18074 (Mouse); 302248 (Rat)
Alternate Names
NID2; NID-2; nidogen 2 (osteonidogen); Nidogen2; Nidogen-2; Osteonidogen

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Citations for Human Nidogen-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A comprehensive proteomics study on platelet concentrates: Platelet proteome, storage time and Mirasol pathogen reduction technology
    Authors: V Salunkhe, IM De Cuyper, P Papadopoul, PF van der Me, BB Daal, M Villa-Faja, D de Korte, TK van den Be, L Gutiérrez
    Platelets, 2018-03-19;0(0):1-12.
  2. Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers
    Authors: GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb
    Cancers, 2021-07-14;13(14):.
    Species: Human
    Sample Types: Tissue Homogenates
  3. Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
    Authors: Smith A, Bowers S, Stratman A, Davis G
    PLoS ONE, 2013-12-31;8(12):e85147.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
    Authors: Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
    Blood, 2009-10-12;114(24):5091-101.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Differential expression of epithelial basement membrane components nidogens and perlecan in corneal stromal cells in vitro
    Authors: Abirami Santhanam, Andre A. M. Torricelli, Jiahui Wu, Gustavo K. Marino, Steven E. Wilson
    Mol. Vis

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