Human Nidogen-2 Antibody Summary
Accession # Q14112
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Nidogen‑2 in Human Kidney. Nidogen-2 was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human Nidogen-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3385) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Nidogen-2 (also named entactin-2) is a 200 kDa, secreted, monomeric basement membrane glycoprotein (1). Nidogens 1 and 2 are expressed in nearly all basement membranes (1‑3) where they interact with laminins, collagen type IV and proteoglycan family members to form structural scaffolds (4, 5). In mouse, Nidogens 1 and 2 appear to substitute for each other. Deletion of one nidogen gives a mild phenotype, but deletion of both nidogens is lethal (6, 7). Affinity of laminin binding is much lower for human Nidogen-2 than that of mouse Nidogen-2, indicating that human Nidogen-2 may not be a strict substitute for Nidogen-1 (1). Both nidogens bind perlecan and collagens I and IV, but only Nidogen-1 binds fibulins (1, 3). The two nidogens show approximately 50% amino acid (aa) identity in human and are structurally similar (1, 4, 6). Cleavage of a 28 aa signal sequence from human Nidogen-2 produces a 1219 aa mature protein containing three globular domains (G1‑3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 Nidogen ( beta -barrel) domain interacts with perlecan, and the C-terminal G3 beta -propeller structure is associated with laminin binding. The mucin-like link region is longer in Nidogen-2 than nidogen-1, and contains both N- and O-glycosylation (2, 8). There is one EGF‑like motif and a short peptide that ligates alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF‑like motifs, two of which bind calcium, and two thyroglobulin type 1 domains that serve as a binding site for alpha v beta 3 integrins. Mature human Nidogen-2 is 80% aa identical to both mouse and rat Nidogen-2, and 73% aa identical to both canine and bovine Nidogen-2.
- Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
- Miosge, N. et al. (2001) Histochem. J. 33:523.
- Salmivirta, K. et al. (2002) Exp. Cell Res. 279:188.
- Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
- Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
- Schymeinsky, J. et al. (2002) Mol. Cell. Biol. 22:6820.
- Bader, B.L. et al. (2005) Mol. Cell. Biol. 25:6846.
Citations for Human Nidogen-2 Antibody
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Citations: Showing 1 - 3
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Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers
Authors: GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb
Sample Types: Tissue Homogenates
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Authors: Smith A, Bowers S, Stratman A, Davis G
PLoS ONE, 2013;8(12):e85147.
Sample Types: Whole Cells
Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
Authors: Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
Sample Types: Cell Lysates
Applications: Western Blot
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