Key Product Details

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 443625
View all NLRP2/NALP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02

Specificity

Detects human NLRP2/NALP2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NLRP2/NALP2 Antibody

Detection of Human NLRP2/NALP2 antibody by Western Blot.

Detection of Human NLRP2/NALP2 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NLRP2/NALP2 Monoclonal Antibody (Catalog # MAB4684) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human NLRP2/NALP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line

Reviewed Applications

Read 1 review rated 3 using MAB4684 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NLRP2/NALP2

NLRP2, also known as NALP2, is a cytosolic 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF‑ alpha production in response to LPS; via procaspase‑1, it promotes proIL‑1 beta cleavage and release. Human NLRP2 is 1062 amino acids (aa) in length. It contains an N‑terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa 109‑132, aa 133‑154 and aa 847‑1062, respectively; a fourth shows a 38 aa substitution for aa 1‑903. No close rodent counterparts have been reported.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

NALP2, NBS1, PAN1, PYPAF2

Entrez Gene IDs

55655 (Human); 232827 (Mouse)

Gene Symbol

NLRP2

UniProt

Q9NX02

Additional NLRP2/NALP2 Products

Product Documents for Human NLRP2/NALP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NLRP2/NALP2 Antibody

For research use only

Citations for Human NLRP2/NALP2 Antibody

Customer Reviews for Human NLRP2/NALP2 Antibody (1)

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  • Human NLRP2/NALP2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 04/13/2017

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