Human NLRP2/NALP2 Antibody

Catalog # Availability Size / Price Qty
MAB4684
MAB4684-SP
Detection of Human NLRP2/NALP2 by Western Blot.
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Human NLRP2/NALP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NLRP2/NALP2 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 443625
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NLRP2/NALP2
Met1-Thr208
Accession # Q9NX02
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NLRP2/NALP2 antibody by Western Blot. View Larger

Detection of Human NLRP2/NALP2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NLRP2/NALP2 Monoclonal Antibody (Catalog # MAB4684) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NLRP2/NALP2

NLRP2, also known as NALP2, is a cytosolic 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF‑ alpha production in response to LPS; via procaspase‑1, it promotes proIL‑1 beta cleavage and release. Human NLRP2 is 1062 amino acids (aa) in length. It contains an N‑terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa 109‑132, aa 133‑154 and aa 847‑1062, respectively; a fourth shows a 38 aa substitution for aa 1‑903. No close rodent counterparts have been reported.

Long Name
NLR Family, Pyrin Domain Containing 2
Entrez Gene IDs
55655 (Human); 232827 (Mouse)
Alternate Names
CLR19.9; FLJ20510; NACHT, leucine rich repeat and PYD containing 2; NACHT, LRR and PYD domains-containing protein 2; NALP2; NALP2NACHT, LRR and PYD containing protein 2; NBS1; NBS1PYRIN-containing APAF1-like protein 2; NLR family, pyrin domain containing 2; NLRP2; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 2; PAN1; PAN1Nucleotide-binding site protein 1; PYPAF2; PYPAF2PYRIN domain and NACHT domain-containing protein 1

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NLRP2/NALP2 Antibody

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Human NLRP2/NALP2 Antibody
By Anonymous on 04/13/2017
Application: WB Sample Tested: Neural progenitor cells Species: Human