|Detection of Human NLRP2/NALP2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NLRP2/NALP2 Monoclonal Antibody (Catalog # MAB4684) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP2/NALP2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
NLRP2, also known as NALP2, is a cytosolic 121 kDa member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NF kappa B, it suppresses TNF‑ alpha production in response to LPS; via procaspase‑1, it promotes proIL‑1 beta cleavage and release. Human NLRP2 is 1062 amino acids (aa) in length. It contains an N‑terminal Pyrin domain (aa 9‑90), followed by a Nacht region and nine LRRs (aa 467‑1033). Four alternate isoforms exist. Three show deletions of aa 109‑132, aa 133‑154 and aa 847‑1062, respectively; a fourth shows a 38 aa substitution for aa 1‑903. No close rodent counterparts have been reported.
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We have 1 review tested in 1 species: Human.We have 1 review tested in 1 application: Western Blot.