Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NLRP3/NALP3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NLRP3/NALP3
Lys540-Lys689
Accession # Q96P20

Specificity

Detects human NLRP3/NALP3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) NALP1, rhNALP2, and rhNALP5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NLRP3/NALP3 (aa 540‑689) Antibody

NLRP3/NALP3 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

NLRP3/NALP3 in THP-1 Human Cell Line.

NLRP3/NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line, unstimulated (lower panel) or stimulated (upper panel) with 50 ng/mL PMA and 200 ng/mL LPS, using Goat Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7010) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human NLRP3/NALP3 (aa 540‑689) Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line, stimulated with 50 ng/mL PMA and 200 ng/mL LPS

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NLRP3/NALP3

NALP3 (NAcht, Leucinerichrepeat and PYD domain containing protein 3; also PYPAF1, CIAS1 and CLR1.1) is a cytoplasmic 115‑130 kDa member of the NLRP family of molecules. It is selectively expressed, being found in stratified squamous and transitional epithelium, Hassell's corpuscles, neutrophils, dendritic cells and T cells. NALP3 promotes IL-1 beta and IL-18 maturation by activating caspase‑1. It does so by forming a NALP3 inflammasome comprised of NALP3, ASC and caspase‑1. Bacterial RNA and cell wall peptidoglycan can bind to NALP3, promoting (potentially) ATP binding, NALP3 oligomerization, and caspase activation. Human NALP3 is 1036 amino acids (aa) in length. It contains an N-terminal DAPIN domain (aa 1-93), a NACHT domain (aa 220-536), and seven consecutive LRRs (aa 740‑991). Alternate splice forms are reported. Either individually, or in combination, there can be deletions of aa 836-892 or 721-777, and a premature truncation after Gly719 that generates a 70 kDa isoform.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Gene Symbol

NLRP3

UniProt

Q96P20 (Human)

Additional NLRP3/NALP3 Products

Product Documents for Human NLRP3/NALP3 (aa 540‑689) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NLRP3/NALP3 (aa 540‑689) Antibody

For research use only

Citations for Human NLRP3/NALP3 (aa 540‑689) Antibody

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FAQs for Human NLRP3/NALP3 (aa 540‑689) Antibody

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Does this NLRP3/NALP3 antibody come in lyophilized form?

    A: Yes, we carry this NLRP2 antibody in lyophilized format.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: An E. coli derived sequence corresponding to Lys540 - Lys689 of human NLRP3/NALP3.

  • Q: Does this NLRP3/NALP3 antibody come in lyophilized form?

    A: Yes, we carry this NLRP2 antibody in lyophilized format.

  • Q: What is the immunogen sequence of this NLRP3/NALP3 antibodies antibody?

    A: An E. coli derived sequence corresponding to Lys540 - Lys689 of human NLRP3/NALP3.

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