Human Nucleoredoxin Antibody

Detection of Human Nucleoredoxin by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Nucleoredoxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5719) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleoredoxin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human Nucleoredoxin by Western Blot

DVL2 and NRX amounts are affected by Ca2+-mediated ROS metabolism.A, Western blots of DVL2 and NRX showing a ROS-dependent increase of both protein amounts occurring 1 h after differentiation or after H2O2 treatment (1 mm). All bands are from the same blot. The signal intensities were normalized to 1 at 0 h (proliferating cells) and quantified as a fold change. B, confocal images of DVL2 (green) and NRX (glow dark) in untreated and 0.5 μm RuR-treated cells at 0, 1, and 3 h of differentiation. RuR inhibits the increase of both proteins (1 h) and removal of RuR allows for the cytosolic accumulation of both proteins (3 h). Mean fluorescent intensities are quantified in bar graphs. n = ∼200 cells per time point. *, p ≤ 0.05. Error bars, S.D. Scale, 10 μm; a.u., arbitrary units. Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S002192582048425X), licensed under a CC-BY license. Not internally tested by R&D Systems.